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Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used.

以巴豆油引起小鼠耳肿胀模型做为抗炎筛选模型,同时,在LC-MS-MS分析的导向下,寻找合欢皮抗炎活性部位。

BACKGROUND: It has been indicated that neuro-immuno-modulation peptide,α-melanophore-stimulating hormone, can play an effective role in anti-inflammation, antibiosis, defervescence and immunological regulation through inhibiting the release of proinflammatory factor, improving the vigor of lymphocyte and other ways, so that it has a good prospect in the prevention and treatment of systemic inflammatory response syndrome.

背景:研究表明,神经免疫调节肽α-黑素细胞刺激素(α-melanophorestimulating hormone,α-MSH)可通过抑制促炎因子释放、改善淋巴细胞活力等途径发挥其抗炎、抗菌、退热和免疫调节作用,在防治全身炎性反应综合征中可能有良好的应用前景。

Objective: To compare the effects of anti-inflammatory and analgesia when the total alkaloid of monkshood processed by a new method is thirteen times higher than that of its small pieces.

目的:比较新法加工附子在总生物碱高于附片13倍时,其抗炎、镇痛的效果是否有差异。方法:通过小鼠热板法、扭体法、小鼠耳廓肿胀法和大鼠足趾肿胀法进行镇痛和抗炎作用比较。

nodular dermal vasculitis in lower extremities are often seen clinically in middle-age women, and are evoked by many causes. Some laboratory examinations have abnormal outcomes and we think these are related with autoimmune reactions of nodular dermal vasculitis. We can make diagnosis with clinical character of erythema and nodose lesions in lower extremities, laboratory examinations and histopathology inspection. Antibiotics, non- Steroid anti-inflammatory drugs, anti-tubercle drugs and corticosteroids are effective drugs.

结节型皮肤血管炎多发于中年女性;结节型皮肤血管炎病因复杂,可能是由于细菌、真菌、病毒、结核、药物等所引起,也可为某些全身性疾病的皮肤表现;实验室指标异常改变可能与疾病免疫反应有关;结节型皮肤血管炎诊断以双下肢红斑结节性损害的临床特点,结合实验室及组织病理学检查可确诊分型;结节型皮肤血管炎针对病因选用抗生素、非甾体抗炎药、抗结核药、皮质类固醇激素等治疗有效。

All the targe compounds had anti-inflammatory activity test. The preliminary bioassay test suggested that some of these compounds should show good anti-inflammatory activity. They are worth investigation in further, and may gradually develop into a new generation of practical anti-inflammatory drugs.

3对所合成的目标化合物进行抗炎活性筛选,发现部分化合物有较强的抗炎活性,值得进一步深入研究,并逐步开发成实用的新一代抗炎药。

Objective: To evaluate the clinical efficacy and safety of diclofenac diethylamine salt gel in the treatment of closed trauma and sprain.

目的 :考察双氯芬酸二乙胺盐凝胶治疗闭合性创伤和扭伤镇痛抗炎的临床疗效和安全性。

(1) Oxidative/antioxidative reactions, inflammatory reactions, apoptosis in lung are involved in the pathogenesis of HILI;(2) ABX, rHuEPO have protective effects on HILI respectively;(3) Hyperoxia can increase VILI in rats, but has little effects with low tidal volume mechanical ventilation.

结论(1)氧化抗氧化失衡,炎症反应,肺内细胞凋亡参与了高氧所致成年大鼠肺损伤的过程;(2)ABX通过抗炎、抗氧化、抑制细胞凋亡作用对成年大鼠高氧肺损伤具有保护作用;rHuEPO通过抗炎、抗氧化、抑制细胞凋亡及一定程度的抗纤维化作用对成年大鼠高氧肺损伤具有保护作用;(3)高浓度氧能明显加重大鼠通气机所致肺损伤,而高浓度氧对小潮气量通气影响较小。

The rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the OD numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Methods ①anti-inflammatory trial: 40 rats were randomly divided into control group, high-dose bushenlizhuo capsule group, middle-dose bushenlizhuo capsule group and low-dose bushenlizhuo capsule group(10 rats in each group).the rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the od numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Objective To establish a HPLC method for the determination of aceclofenac in human plasma.Methods Acidified samples of plasma (500μL) were extracted with 4 mL of ethyl acetate. The organic phase was evaporated to dryness by nitrogen at 40℃ water bath. The residue was redissolved in mobile phase and then was determined.

醋氯芬酸是一种具有镇痛作用的非甾体类抗炎药物[1],化学名为[邻-(2,6-二氯苯基)氨基]苯乙酰乙酸,l992年首先于西班牙上市,具有明显广泛的抗炎作用和强力的解热镇痛作用,其生物半衰期较双氯芬酸长,胃肠耐受性较双氯芬酸好,抗炎活性优于萘普生和保泰松[2]。

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