抗溶菌的

After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including ESTss analogous to 11.5KD antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. Primers designed according to the sequences were used to amplify the corresponding ESTss from both blood and cephalothorax cDNA library.

在仔细分析了它们在免疫系统中的重要性和在对虾中出现的可能性之后，从中选出了17条可能编码抗菌肽，溶菌酶，凝集素、丝氨酸蛋白酶及其抑制剂，抗冻蛋白等蛋白质的序列，以此为依据设计引物，在中国对虾的血液和头胸部cDNA文库中扩增相应的序列。

The results show that the synthetic compounds conform to the target compounds and the primary bioassay for anti-vibrio activity displays that the pyrrole alkaloids have a weak inhibition against Vibrio parahaemolyticus and Vibrio alginolyticus compared with the positive control of chloromycetin.

结果表明，所合成的化合物与目标化合物结构相一致。初步的抗溶藻弧菌和副溶血弧菌活性筛选表明，合成的吡咯生物碱具有一定的抗弧菌活性，但相对于阳性对照药氯霉素来说，合成的绝大部分吡咯生物碱抗弧菌活性较弱。

In this paper, the ability and mechanism of antibacterial and immune defense ofChinese shrimp was studied using cytological, biochemical, molecular biological andproteomic methods. A sensitive and stable antibacterial and lysozyme assay methodswere selected. The effect of Vibrio anguillarum and laminarin (a kind of beta-1,3-glucan) on the immune ability of haemolymph of Chinese shrimp was analyzed.The changes of total haemocyte counts, antibacterial ability, lysozyme activity andlysosome membrane stability were detected.

本文采用细胞学，生物化学，分子生物学和蛋白质组学等技术，对对虾的抗菌能力和免疫防御机制进行研究，筛选了灵敏稳定的抑菌和溶菌检测方法；并对灭活鳗弧菌和海带多糖(laminarin，一种B-1， 3 葡聚糖)免疫诱导后的中国明对虾血细胞数目，血淋巴的抑菌活力，溶菌能力，溶酶体膜的稳定性进行分析；对两种重要免疫因子溶菌酶和精氨酸激酶进行克隆和表达分析。

The culture of Micrococcus aureus in lag phase and decline phase are more sensitive to TFP than that of in logarithmic phase and stable phase.

抗菌蛋白对金黄色微球菌表现出杀菌作用，但无溶菌现象；在金黄色微球菌的四个生长阶段中，延滞期与衰亡期菌体对抗菌蛋白的敏感性强于对数期及稳定期。

By adopting single-factor and orthogonal tests, the optimum hydrolytic conditions of razor clam such as enzyme concentration, hydrolysis temperature, reaction time, substrate concentration and pH value wore determined with the degree of hydrolysis as the main index.

溶菌酶、NISIN等混合使用比单独使用保鲜效果更佳，可能因为各自的适用条件及抗菌范围存在一定的互补性，因而扩大了其抗菌谱，这对延长缢蛏冷藏保鲜货架期具有一定的实际意义。

The bacteriolytic channel formed from hyphal self-digestion was found in the area of dark line barrage by microscopic observation, while no hyphal self-digestion was found in the other two barrage types.

显微观察暗线型拮抗线区域有菌丝自融形成的溶菌沟，另2种拮抗线中没有菌丝自溶。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

Analysis and fitting of growth curves in Liyang chicken;2. The results of antibacterial spectra and growth curves of eleven strains with better antagonistic effect showed that: 4 strains isolated from NAC plate had wider range of antibacterial spectra,3 strains isolated from manganic nutrient agar plate had advantage on growth.

用点种法从大黄鱼肠道优势菌群中筛选出104株病原性副溶血弧菌的拮抗菌，进一步研究拮抗效果最好的11株拮抗菌的抗菌谱和生长曲线，其中NAC平板上筛选的4株拮抗菌的抗菌范围较广，锰营养琼脂培养基上筛选的3株拮抗菌具有生长优势。

Comparing with chicken egg-white lysozyme which is widely used in clinic, hLYZ has many advantages: it is a natural protein in human body, so it is safe and harmless when used as a drug; its bioactivity to kill bacterium is three times higher than chicken egg-white lysozyme, and its thermal stability is much higher, too; it also has many other specific important functions which are not related with its catalysis, such as anti-virus and anti-tumor and improving immunization.

人溶菌酶与目前临床上应用最多的鸡蛋清溶菌酶相比具有以下优势：人溶菌酶是人体内的蛋白，具有天然的相容性，没有毒性和副作用；人溶菌酶比鸡蛋清溶菌酶的杀菌活性高3倍，而且热稳定性也要远高于鸡蛋清溶菌酶；人溶菌酶还有多种特有的与其自身的催化作用无关的理化作用，如抗病毒和抗肿瘤。

Lysozymes were immobilized on wool fabrics by sol-gel method in order to enhance the antibacterial function of wool.

为了增强羊毛织物的抗菌性，采用溶胶－凝胶法将溶菌酶固定在羊毛织物上，以期获得良好的抗菌效果。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失