抗淋巴细胞血清

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

Objective To explore the inhibition of Pseudomonas aeruginosa exotoxin A on lymphoproliferation and neutralization of anti-PEA serum.

目的 探讨铜绿假单胞菌外毒素A对人淋巴细胞转化的抑制作用及抗－PFA血清的中和作用。

The model had a high incidence and its pathologic changes were close to those of multiple sclerosis, so it might be an ideal model for the research of MS. 2. It is supposed that the activity of T cell response to MOG_(35-55) and the increases of anti-MOG_(35-55) antibody and IFN-γlevels may play key roles in the onset of EAE.

通过比较EAE小鼠和正常小鼠T淋巴细胞的增殖、血清抗MOG_(35-55)抗体和IFN-γ水平的不同，显示T细胞的活化、抗体的升高、细胞因子的异常可能共同导致了EAE的发生。

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性，进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠，3次免疫小鼠后，血清(1：1000)中能检测到较强的MUC1抗体阳性；以最大OD450值之纯化血清做为一抗行蛋白印迹分析，结果显示：纯化血清可识别MUC1蛋白；小鼠脾脏淋巴细胞增殖率检测结果表明：噬菌体展示肽表位可在无佐剂存在情况下，大大促进小鼠的脾淋巴细胞的增殖，证明噬菌体是一较好的生物载体。

This study concludes the following four parts:(1) acute toxicity of several chloroanilines to zebra fish;(2) effects of chloroanilines on antioxidant defense of crucian carp;(3) effects of chloroanilines on serum sex steroids in crucian carp;(4) effects of chloroanilines on lymphocyte proliferation in crucian carp, and the detailed results and main conclusions are concluded as follows

本论文以2-氯-4-硝基苯胺、4-氯-3-硝基苯胺、2-氯-5-硝基苯胺、2，4-二氯苯胺、3，4-二氯苯胺、2，5-二氯苯胺、2，3-二氯苯胺为研究对象，研究分为四部分：(1)氯代苯胺对鱼类的急性毒性；(2)氯代苯胺对鲫鱼抗氧化系统的影响；(3)氯代苯胺对鲫鱼血清性类固醇激素水平的影响；(4)氯代苯胺对鲫鱼淋巴细胞增殖的影响。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

The blood samples were collected at day 1 before and after the caprine placenta peptide were given for 10 days, and the number of the white blood cell and the ANAE+ in periperal blood ,the content of the globulin, the activity of lysozyme, the T-Aoc ,the activity of the GSH-Px and SOD, and the content of the MDA in serum were determined .

在口服羊胎盘肽的前1d和连续口服羊胎盘肽10d后的第1d各组采血样一次，测定外周血白细胞数、ANAE+淋巴细胞百分率、血清球蛋白含量、血清溶菌酶活性、血清总抗氧化能力、血清谷胱甘肽过氧化物酶活性、血清超氧化物歧化酶活性及丙二醛含量。

The immuneregulating action that briefed on the electropuncture further regulated each other throughthe neural peptide / neurotransmitter, hormone and CK. 6Function laws, which compare point: Dazhui is good at suppressing theinflammation, while Mingmen is strong to the regulating immune function; it is Feixuethat has regulating action too.

比较腧穴的免疫调节作用规律：大椎穴的抗炎治疗作用(如足爪肿胀率、下丘脑 CRH 含量，血清 TNFα含量)较强，命门穴对于免疫调节作用(如脾脏 T、B 淋巴细胞转化率、IL-2 活性、肾上腺 IL-2 含量等)较强，非穴也具有一定的抗炎免疫作用，但弱于大椎穴及命门穴。

The results showed that capsule enhanced the ability of lymphocyte multiplication and macrophagocyte phagocytosis,and also increased the number of plague forming cell and the titers of hemagglutination against sheep red cell at 250 and 750 mg/kg BW.

实验结果表明，250和750 mg/kg剂量的富硒胶囊可提高小鼠淋巴细胞增殖能力、单核巨噬细胞吞噬能力、脾脏抗体形成细胞数和血清中抗绵羊红细胞抗体效价。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。