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抗氧化酶

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The blood samples were collected at day 1 before and after the caprine placenta peptide were given for 10 days, and the number of the white blood cell and the ANAE+ in periperal blood ,the content of the globulin, the activity of lysozyme, the T-Aoc ,the activity of the GSH-Px and SOD, and the content of the MDA in serum were determined .

在口服羊胎盘肽的前1d和连续口服羊胎盘肽10d后的第1d各组采血样一次,测定外周血白细胞数、ANAE+淋巴细胞百分率、血清球蛋白含量、血清溶菌酶活性、血清总抗氧化能力、血清谷胱甘肽过氧化物酶活性、血清超氧化物歧化酶活性及丙二醛含量。

Adopt poaching and alcohol sinking to get coarse polysaccharide, then take MCP, MCP-1 and MCP-2 for studing in antioxidation activities. They have strong ability for scavenging free radical only in DPPH system and oxidation system of linolic acid induced by alkane radical, but negatively in hydroxyl system and in super-oxygen anions system. And MCP-2 is better than MCP-1, so we choose enzymolysis method to remove protein.

采用水煮醇沉工艺提取粗多糖,然后对MCP、MCP-1和MCP-2进行抗氧化活性试验,三者在DPPH体系和烷基自由基引发的亚油酸氧化体系中具有明显的清除自由基能力,在羟基自由基体系和超氧阴离子自由基体系中则呈现负清除能力,其中MCP-2要优于MCP-1,因此选用酶解法除蛋白。

The rats of model group,acupointⅠgroup, acupointⅡgroup were treated with passive smoking.The rats of acupointⅠgroup,acupointⅡgroup were treated with placing Chinese medicine at the point of feishu,pishu for 5 hours or 10 hours after depilation.

治疗结束后观察支气管组织病理特征及细胞浸润情况,并对其进行气道炎症病理评分;酶联免疫吸附法测定被动吸烟大鼠肺泡灌洗液中α_1-抗胰蛋白酶、TNF-α、IL-8含量;黄嘌呤氧化酶法测定被动吸烟大鼠肺泡灌洗液SOD的活性、TBA法测定MDA的含量。

Ultrastructural observation showed that there was a great deal of damage by Cd2+ pollution:The thylakoids of chloroplast and cristae of mitochondria became swollen or vesiculate and disintegrated finally;The nucleus were distorted and the nuclear membrance was disrupted.

但随着毒害的加重,当超出细胞的保护限度,将会导致保护酶活性降低,抗氧化物质含量下降,而丙二醛等膜脂过氧化产物大量产生,膜透性增加,使植物体受害加剧。

Free radical hurts biological large molecules, lets lipid peroxid- ize and damages membrane, the weakening of antiperoxidation system in vivo (vit E, SOD, GSH-PX) will increase the harmful effect of radical on cells.

自由基损伤生物大分子,使脂类过氧化,造成膜损伤;机体抗氧化体系(维生素E,超氧化物歧化酶,谷胱甘肽过氧化物酶等)作用的削弱会加重自由基的细胞毒作用。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase Ⅱ detoxifying genes.

在正常情况下,Kelch样环氧氯丙烷相关蛋白-1(Kelch-like ECH-associated protein-1, Keap1)与Nrf2耦联,并与肌动蛋白细胞骨架结合被锚定于胞浆,但是在半胱氨酸残基发生氧化的情况下,Nrf2和Keap1解耦联,进入细胞核并与ARE结合,从而激活多种抗氧化基因和Ⅱ相解毒酶基因的转录。

Researchers in University of California and George Washington University have found that barley grass juice contains the potent antioxidants superoxide dismutase, 2"-O-glycosylisovitexin (2"-O-GIV) and vitamin E succinate, many amino acids and numerous enzymes.

加州大学及乔治华盛顿大学的多位教授都发现大麦苗汁含有强力的抗氧化物:超氧化抗突变酶、2-O-GIV、维他命E succinate及丰富的叶绿素等。

PL could maintain cellular membrane integrity,induce the oxidative damage to biomembrane by free radicle and improve antioxygen ability and ATPase activity of larval fish.5 Effect of phospholipid supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvaePurpose:This study was undertaken to determine the effect of commercial PL supplementation in formulated microdiets on foregut and liver histological changes of Pelteobagrus fulvidraco larvae,which can further investigated the mechanism of the growth-promoting effect of PL with the study of histology and digestive physicalogy.

磷脂能够维持黄颡鱼仔稚鱼细胞膜的完整性,降低自由基对生物膜的过氧化损伤,提高机体抗氧化系统的活力和ATP酶活性。5饲料中不同磷脂添加水平对黄颡鱼仔稚鱼前肠和肝脏组织学的影响目的:研究在实际配方的人工微粒饲料中添加商品大豆磷脂对黄颡鱼仔稚鱼消化肠道肝脏器官发育及脂肪消化吸收的影响,进一步从组织学消化生理角度揭示磷脂对仔稚鱼促生长作用机理。

At the early stage of stress (2 d and 5 d), the Pn, Tr, Ci and Gs of own-root plant were significantly decreased, while Ls was significantly increased, the inhibition of photosynthesis of own-root plant was mainly caused by stomatal factor, water use efficiency was significantly elevated, resulting from lower Pn.

在NaCl胁迫下,自根苗和嫁接苗叶片和根系中酶(SOD、POD、CAT和APX)活性和抗氧化剂含量发生明显变化,或被诱导上升,或被抑制下降,但嫁接苗各指标均显著高于自根苗,而丙二醛含量显著低于自根苗,表现出较强的抗氧化能力。

After seedling trees of Yunhe Snow Pear were polluted by Pb(superscript 2+), Cd(superscript 2+), synthesis of chlorophyll was restrained, toxic effect of Pb(superscript 2+), Cd(superscript 2+) on seedling trees was intensified, membrane lipid peroxidation damage was increased. And the protective enzymes system start-up of polluted seedling trees enhanced antioxidative enzymes activities of seedling trees.

结论]Pb(上标 2+)、Cd(上标 2+)污染云和雪梨苗,抑制叶绿素的合成,增强超养阴离子自由基O2(上标-对植株的毒害性,使膜脂过氧化损伤程度高,受重金属污染植株启动保护酶系统,以增强植株的抗氧化性。

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