抗氧化酶

Ratio of enzyme to substrate, temperature, and hydrolysis time for the production of antioxidant peptides were analyzed with Response surface methodology in which reducibility and superoxide anion radical capturing rate was response value to build a predictive model for the enzymolysis of chickpea protein and the optimization of the enzymolysis technology parameters.

为优化Alcalase蛋白酶酶解鹰嘴豆蛋白制备抗氧化肽的工艺条件，采用响应面分析法（response surface methodology，简称RSM），以还原能力、超氧阴离子捕获率为响应值，研究了酶与底物的比值、酶解温度和酶解时间对制备抗氧化肽工艺的影响。

The anti-oxidant enzymes including SOD, POD and CAT and MDA, which is one of the oxidant products, and IAA and ABA, which were relative to earliness but not to decrepitation of the short-season upland cotton, were studied.

为解决棉花育种中普遍存在的早熟早衰问题，研究了与短季棉早熟不早衰有关的抗氧化系统酶包括超氧化物岐化酶SOD、过氧化物酶POD 和过氧化氢酶CAT，氧化产物之一丙二醛性MDA，以及生长素IAA 和脱落酸ABA在不同类型短季棉品种中不同生长发育时期的变化规律和遗传特性。

In currence, se- GSH- PX activity have been been used as a standard of Selenium nourishment, GSH- PX is an extremely important protection enzyme in anti oxida- tive defend system of organize and cell of animal.It clear hydroxy radical and hyperoxide in cell respir- ation metabolism reaction ,thereby, mai- ntain cell's normal physiological function, prote- ct biomembrane and biomacromolecule from oxidative damage.If the activity is high or not, effect the ab- ility that the organize clears hyperoxize lipi- d ,directly.

目前，含se-GSH-PX活性已被作为se营养状况可一个指标，该酶是动物组织细胞抗氧化防御系统中一个极为重要的保护性酶，它是以还原型GSH为底物的谷胱甘肽参与的过氧化反应，清除细胞呼吸代谢反应过程产生的羟自由基和过氧化物，从而使细胞能维持正常的生理功能，保护生物膜和生物大分子结构免受氧化损伤。

The results indicate seawater concentration has no effect on absorbency, pH, relative density, dry matter and water contents in Aloe vera leaves. While, seawater stress causes increased MDA and membrane penetration, decreased phytochroms, prolines and NO_3~--N. It is beneficial to plant growth when irrigation was done with 10% seawater, because of higher ratio of K~+/Na~+ and lower ratio of Na~+/Ca~(2+). In addition, the polysaccharide contents in Aloe vera leaves increase twice and triplication under 25% and 50% seawater irrigation respectively, the barbaloin contents increase over triplication in var. 75% seawater and over twice in var. 50% seawater and var. 100% seawater. However, when seawater concentration exceeds 25% the growth of plant was inhibited, biomass decreased, flowering time was deferred, Ca~(2+) content decreased caused by Na~+ accumulation in leaves.

结果表明：在海水胁迫下，库拉索芦荟全叶汁的吸光度、酸度、相对密度、总固形物和水分含量总体上受海水浓度的影响不大；但芦荟叶的丙二醛含量和质膜透性增加，叶绿体色素、脯氨酸和硝态氮含量减少；在10％海水浇灌下，芦荟叶细胞具有较高的K~+／Na~+比和较低的Na~+／Ca~(2+)比，对库拉索芦荟的生长有一定的促进作用；在25％海水和50％海水浇灌下，芦荟多糖含量提高了2-3倍；在75％海水浇灌下芦荟甙含量增加了3倍多，在50％海水和100％海水灌溉下，其含量也增加了2倍多；当海水浇灌浓度超过25％后，库拉索芦荟的生长明显受到抑制，生物量降低，抽苔期推迟，同时，海水浓度的增加也导致芦荟叶中累积大量Na~+，从而使得芦荟叶中Ca~(2+)含量降低，但Mg~(2+)并没有受到海水浓度的影响；用浓度低于75％的海水浇灌库拉索芦荟可以降低剖面深度在0-100cm之间的土壤盐度；其抗氧化保护酶中超氧化歧化酶的酶活力相对保持稳定，过氧化物酶的酶活力在10％海水浇灌下明显提高，随后则下降，而过氧化氢酶的酶活力则随海水浓度的升高而下降。

In this paper, on the basis of a brief introduction of cotton enzymatic antioxidant system, the research progress on enzymatic antioxidant system response to different stresses, such as drought, salt injury, verticillium and so on, in recent years were reviewed.

本文试图以对棉花的酶促抗氧化系统的简单介绍为基础，综述棉花酶促抗氧化系统对干旱、盐害和黄萎病等逆境胁迫的生理响应的研究进展，分析目前相关研究中存在的问题及其研究前景。

Wenxin capsule can promote endolethial cells releasing CO and synthesing 〓, enhance activity of HO, induce angiectasis through activating guanylic cyclase and adenglate cyclate of intracellar of vessel smooth muscle cell to enhance content of intracellar cGMP and AMP. 3. Wenxin capsule can inhibit releasing of platelet activiting factor 〓 which inhibit platelet aggregation and have antithrombosis function. 4. Wenxin capsule can promote activity of t-PA, reduce activity of PAI-1, strengthen fibrinolytic function, therefore inhibit fibrin disposition. 5. Wenxin capsule can inhibit synthesis of smooth muscle cellular DNA. Its function don't kill it directly but prevent transformation from period Go to peried S. 6. Wenxin capsule is an effective prescription of anti-coronary atherosclerosis atherosis. The results of image pattern analysis tastifies that area of lipid plaque in treating group reduce 54% and 58% in contrast with that in control group. The results of light, electro- microscopy show that structure of endolethial cell and smooth muscle cell in treating group is more integral than that in control group.

其作用机制之一是由于该药具有良好的调脂降脂作用，其不仅能明显降低血清LDL-c及升高HDL-c含量，且能有效阻止TC沉积于血管壁，从而保护内皮细胞结构完整；此外，温心胶囊尚具有明显的抗氧化作用，该药不仅能明显降低血浆LPO含量，且能提高SOD活性，同时其对血红素在细胞内代谢的调节作用也参与了抗氧化过程。2 温心胶囊能明显促进内皮细胞释放CO及合成〓，提高HO活性，通过激活血管平滑肌细胞内鸟苷酸环化酶及腺苷酸环化酶引起细胞内cGMP、AMP浓度增加而引起血管舒张。3 温心胶囊能明显抑制血小板激活因子〓的释放，从而抑制血小板聚集，抗血栓形成。4 温心胶囊能明显提高t-PA活性，降低PAI-1活性，增强纤溶功能，从而抑制纤维蛋白沉积。5 温心胶囊能明显抑制平滑肌细胞DNA合成，其抑制作用不是直接杀伤抑制，而是阻止细胞由Go期向S期转化。6 温心胶囊是抗冠状动脉粥样硬化的有效制剂，图象分析结果证实，该药治疗组脂斑面积比对照组缩小了54％和58％，光电镜结果显示内皮细胞及平滑肌细胞结构较为完整。

NB can make liver, brain, spleen and nephridial tissue pathology damage, to refrain theactivity of liver mixed function oxidase, induce oxidative stress, effect tissue enzymatic activity,destroy organism antioxygen defence system and function of clean up free radical, to cause proteinoxidation damage, to educe toxic action.

综上所述，NB可以造成肝、脑、脾、肾组织的病理学损伤，抑制肝内混合功能氧化酶的活性，诱导氧化应激，影响组织酶活性，破坏机体抗氧化防御系统及清除自由基功能，引起机体蛋白质氧化损伤，进而发挥其毒性作用。

In the cardiovascular system, the endogenous NO, CO, and H2S form three specific and relevant pathways with their corresponding enzymes including nitric oxide syntheses, the home oxygenize and cystathionine-r-lyric enzyme, whose affect can relieve the atherosclerosis.

血管系统中内源性气体信号分子NO、CO、H2S作为一种独特的血管活性物质，分别与其相应的合成酶一氧化氮合酶、血红素加氧酶和胱硫醚－r-裂解酶形成独立而又相互关联的体内重要抗氧化系统，可以减少动脉粥样硬化的严重度。

A pot experiment was conducted to investigate the effect of soil water on protective enzymes of superoxide dismutase, catalase and peroxidase, and also on membrane lipid peroxidation including malonaldehyde and plasma membrane permeability in pea (Pisum sativum Linn.) leaf during flowering and pod-bearing stages under drought stress and water recovery conditions. Antioxidant ability of pea during flowering and pod-bearing stages was also analyzed.

为探讨不同水分条件对豌豆保护酶系统和膜脂过氧化的影响，采用盆栽人工控水试验方法，模拟干旱胁迫及复水条件，研究了不同水分处理对花荚期豌豆叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶活性以及过氧化产物丙二醛含量和脂膜相对透性的影响，并对花荚期豌豆抗氧化能力进行了综合评价。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针，筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库，得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase，SO)有60～90％的同源性，长度为647bp，编码140个氨基酸，具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20～40％的同源性，长度为1047bp，编码279aa，是一个不完整的开放阅读框，预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80～90％的同源性，长度为1810bp，编码493aa，具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30～70％的同源性，长度为1446bp，具有开放阅读框和BTB／POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框，与玉米Cat-3基因具有81％同源性，预测R8-1-1可能具有与Cat-3类似的功能，可为转基因小麦抗逆育种提供新的基因。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。