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By the treatment of PMA /Ionophore or crosslinking CD16 molecules, Fas ligand molecules were induced, Fas molecules were with same expressive pattern but the expressive amounts of LTb and LTbR molecules were significantly reduced to 30-50% of the original ones.

之后在PMA/ Ionophore或抗CD16抗体的刺激下, Fas ligand被表达出来, Fas的表现量不变,而LTb和LTbR的表达却降为原本的30-50%。

Methods:The peptide sequences of human CYP1A2 were analyzed according to its bioinformatics, and synthesized on the basis of hydrophilicity, antigenicity, accessibility and flexsibility. The synthesized peptides were crosslinked with keyhole limpet hemocyanin, which was used to immunize rabbits for production of Ab.

利用生物信息学方法分析CYP1A2蛋白的序列,根据亲水性、抗原性、柔韧性及表面性等指标选择多肽序列,合成CYP1A2多肽,与载体蛋白钥孔戚血蓝素(Keyhole limpet hemocyanin, KLH)偶联,免疫日本大耳白兔制备抗CYP1A2抗体。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列,利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列,应用Blast软件对所选序列进行同源性分析并合成多肽,与载体蛋白钥孔戚血蓝素偶联,免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

The six McAbs against the Vn of H. longicorns prepared and identified successfully in this study may provide an important tool for further elucidating vitellogenesis and its regulation mechanism in H. longicorns.

本研究成功制备了6株抗长角血蜱Vn的单克隆抗体,为深入阐明长角血蜱卵黄发生的过程与调控机理提供了重要的工具。

Coli, and purify rWHcAg under native condition by precipitation with saturated ammonium sulfate and CsCl gradient centrifugation; Immunize Balb/c mice with purified rWHcAg to prepare antiserum; Establish a competitive inhibition ELISA method to detect WHcAb in serum of Chinese marmots.

原核表达重组WHcAg,氯化铯密度梯度离心获得非变性的纯化蛋白;用该纯化蛋白免疫Balb/c小鼠制备多克隆抗体;建立竞争抑制ELISA方法用于检测旱獭血清中的抗-WHc。

To investigate the epidemiology of hepatitis E virusinfection in macaques,rats,marmots,pigeons,chickens,fish and toads.

目的了解猕猴、鼠、旱獭、鸽子、鸡、鱼、蟾蜍血清中抗-HEV抗体流行情况。

Objective To investigate the pattern of anti-Fas monoclonal antibody-induced apoptosis and its role in treatment of lung cancer.

目的 探讨抗Fas单克隆抗体诱导肺癌细胞凋亡的规律及在肺癌治疗中的意义。

Monoclonal antibody and polyclonal antibody were obtained, and some properties of them were identified respectively.

此外,应用这两种抗体建立hBLyS的双抗夹心ELISA检测方法。

199Au-labelled monoclonal antibody against cancer may be applied to the diagnosis and therapy of tumors.

~(199)Au标记的抗肿瘤单克隆抗体,适用于肿瘤的诊断和治疗。

Objective: To prepare monoclonal antibody against human PCIA1 protein and study its biological activity.

目的:筛选制备抗人PCIA1的单克隆抗体,鉴定其生物学特征。

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This one mode pays close attention to network credence foundation of the businessman very much.

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