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Purified proteinwas dialysed and freezed-dry to inject rabbit preparing rabbit anti-SMOR28 antibody.

利用Ni-NTA琼脂糖提纯表达的目的蛋白SMOR28,SMOR28经透析除盐,冻干浓缩后,免疫兔子制备其抗兔多克隆抗体。

Methods CCL20 and HBsAg eukaryon expression vector was constructed by using DNA recombinant technology.

方法采用基因重组技术,构建趋化因子CCL20和表面抗原的真核表达载体,将重组载体用肌肉免疫的方式分别注射正常的C57BL/6小鼠,通过ELISA方法检测C57BL/6小鼠的抗-HBs抗体水平、细胞增殖情况。

AIM: To prepare and identify the monoclonal antibody specific for the germ tube of Candida albicans.

目的制备抗白念珠菌芽管的单克隆抗体mAb03.2C1-C2,并对其特性进行分析,以探讨其应用于实验室检测的可行性。

Inhibitory effect of natural monoclonal anti??keratin antibody IgM 3B4 on germ tube formation and adhesion of Can??dida albicans in vitro AIM: To investigate inhibitory action of a natural monoclonal anti??

目的:观察单克隆天然抗角蛋白抗体IgM 3B4在体外抑制白念珠菌芽管形成及抑制白念珠菌与宿主细胞粘附的作用。

Different size of the colloidal gold particle was prepared by reducing the gold chloride with sodium citrate heated by microwave oven.

采用柠檬酸三钠还原氯金酸法,用微波炉加热,制备了不同粒径的胶体金,以17.5nm左右的胶体金标记抗克百威多克隆抗体的显色效果较好。

Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make colloidal gold were labeled with monoclonal antibody against SM and were coated onto the glass fiber after the mixure veered purified.

采用柠檬酸三钠还原法制备胶体金,胶体金标记抗体经纯化后吸附在玻璃纤维素膜上;将Str-OVA和羊抗鼠IgG分别包被在硝酸纤维素膜上。

Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated. A series of generic haptens were synthesized and coupled to bovine serum albumin and ovalbumin for immunogens and coating antigens, respectively. New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.

通过分析硫代磷酸二乙酯类农药的结构特点,设计并合成了系列半抗原;采用活泼酯法将半抗原分别与牛血清蛋白和卵清蛋白偶联制备了系列免疫原和包被原;通过免疫新西兰大白兔获得了相应抗硫代磷酸二乙酯类农药的类特异性抗体。

Abstract] Objective To study the fact that influenced the anti-A antibody of hybridoma.

摘 要]目的研究影响抗A杂交瘤细胞抗体分泌的部分因素。

Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

通过试管法和玻片法检测抗A抗体的效价和亲和力。

Objective Preparation of anti-human BPI23 monoclonal antibody with hybridoma technique and preliminary analysis of the application.

目的 采用杂交瘤技术制备抗人BPI23单克隆抗体,并对其应用进行初步分析。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。