抗抗体

While the positive immunofluorescent staining of cenexin could only be seen in one of the centrioles, suggesting that the cenexin protein might be a special component of human matured sperm centrosome.

值得注意的是，利用抗Centrin多克隆抗体对18例男性不育病人精子的免疫荧光观察发现一些精子中Centrin蛋白的分布异常，主要表现为Centrin弥散，未显示正常大小的近端和远端中心粒。提示中心体蛋白异常是否为引致某些男性不育症的因素之一，值得进一步研究。

Objectives 1 To explore the type, distribution, quantity, and clinical significance of auto-antibodies on bone marrow hematopoietic cells in patients with immunorelated pancytopenia; 2 To evaluate the sensitivity of bone marrow mononuclear cell Coombs tests.

目的 (1)了解免疫相关性全血细胞减少症患者骨髓造血细胞结合的自身抗体的类型、分布、数量及临床意义(2)考察骨髓单个核细胞直接抗人球蛋白试验(BMMNC-Coombs)的敏感性。

After renal cortexes were Grinded and digested with 0.25%tryspase.The cell types of epithelial cell were identified by immunocytochemistry.The injury model of rats, renal tubular epithelial cells were established by using gentamicin.And observed the role of bFGF in renal tubular epithelial cell.

通过对大鼠肾皮质研磨、过网的方法，并以0.25%胰蛋白酶消化，以免疫细胞化学方法鉴定体外培养的肾小管上皮细胞(抗Cytokeratin 18抗体)，以庆大霉素对培养的肾小管上皮细胞建立损害作用模型，然后观察碱性成纤维细胞生长因子对损害的肾小管上皮细胞的保护作用。

Before and after the treatment with corticoid plus small dose of heparin for 4～6 weeks,the serum ACA was measured by ELISA method in 20 nephrotic syndrome children and the results were compared with those obtained from 40 normal children.

近年来有研究报道抗心磷脂抗体( anti- cardiolipin antibody，ACA)在成年人原发性肾病综合征的发病及发展中可能有着重要的作用，但在儿童原发性肾病综合征小剂量肝素钙治疗前和治疗后 ACA检测其高凝状态未见报道。

Cervical mucus, semen consistency pilot test of time in the prediction of ovulation election period, a drop of glass on the first and fresh semen, cervical mucus and then take a drop of semen on the next, distance 2,3 mm, not to coverslip pressure to hand light light rock slide, so that two drops of liquid close to each other, under the microscope to observe sperm penetration capacity, such as the sperm can move through the mucus and to continue to run that sperm motility and cervical mucus of the characters are normal, mucus-free anti - sperm antibodies.

宫颈粘液、精液相合试验试验时间选在预测的排卵期，在玻璃片上先放一滴新鲜精液，然后取子宫颈粘液一滴放在精液的旁边，距离2、3mm，不要盖玻片加压，以手轻轻摇动玻片，使两滴液体互相接近，在显微镜下观察精子的穿透能力，如精子能穿过粘液并继续向前运行，表示精子活动力及宫颈粘液的性状都正常，粘液中无抗精子抗体。

The coverslip with differentiated cells were fixed at different timepoints and used for toludine blue or inmunohistochemical dying Meanwhile, different doses of antibody to Fibronectin were added to observe aggregating phenomenon.

用2，3传代细胞按一定比例种植于24孔板内，加入TGF-β1 1，5，10ng／mL，分别于不同时间点取材固定，用于甲苯胺兰组织学染色及抗纤维连接蛋白抗体免疫组化染色。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

The number of platelet antibody crossmatch coincidence in 112 specimens of AA, 42 specimens of MDS and 95 specimens of leukemia were 45, 20 and 40, the coincidence rates were 40.18%、47.62% and 42.11%.

究其原因--血小板输注无效主要是免疫性破坏，这是当前输血领域内所关注的焦点之一。引起免疫性血小板输注无效的原因首先是HLA抗原不合，其次是血小板特异抗原的抗体，常见的有抗HPA2b等。

Historically, the pretransplant T-cell crossmatch and antibody screening were done by anti-human globulin-complement-dependent cytotoxicity.

先前，采用抗人球蛋白补体依赖的细胞毒性试验进行移植前T细胞交叉配型以及抗体屏蔽。

blood serums of the crowd are collected, antibody corresponded to the four virus examined by elisa、pa and colloidal gold strip. western blot is the confirmation test of positive for antibody of hiv. result: in the 3852 cases of paid blood donor there are 7 curriers of hiv are detected and they are all paid blood donor in past.

采集有偿献血员的血清，通过酶联免疫吸附实验、明胶颗粒凝集实验和胶体金实验来检测血清中的这四种传染病病原体的相应抗体，抗－hiv阳性确认实验是蛋白印迹实验。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。