抗抗体

Recombinant proteins could be considered as SARS-CoV vaccine especially as the vaccine component, which redounds to the development of a potential DNA vaccine.

构建的DNA疫苗可以在小鼠体内产生抗SARS的特异性抗体，为SARS-CoV DNA疫苗的开发提供借鉴作用。

Methods: The human Fd repertories genes were amplified by RT-PCR from PBMC of hepatoma patients, and cloned into the vector pComb3X with chimeric L chain to construct the human-mouse hybrid Fab phage library.

利用RT-PCR自肝癌患者外周血淋巴细胞中扩增全套人抗体重链Fd基因片段，克隆入含嵌合L链的展示载体pComb3X，建立人－鼠杂合Fab噬菌体抗体库。

Results: In HPC and Z n2+ induced groups, all the contents of MT and the activities of antioxida ses were obviously higher than those in H/R (P.05,P.01 respectively ). While using MT, the activities of SOD were progressively increased than contr ol(P.05), CAT and GSHpx activities were lower than control(P.01), bu t were significantly higher than those in I/R group(P.01). In MT antibody g roups, the activities of antioxidase were decreased extremely.

结果：HPC和锌诱导组MT含量及抗氧化酶的活力均显著高于H／R组P.05、P.01：外源MT处理后 SOD活力显著高于对照组（ P＜0.01，CAT和GSHpx活力虽然显著低于对照组但显著高于H／R组(P＜0.01)；使用M T抗体后，酶活力最低。

Methods RF was measured by the rate scatting immunity method.

方法对 RA患者 12 7例、非 RA其他风湿病患者 10 2例及正常对照组 4 3例，采用速率散射免疫比浊法检测 RF；酶联免疫吸附法定量检测抗- CCP抗体；免疫荧光法检测 AKA、APF，并采用四格表法计算敏感度及特异性。

Objective To establish a hybridoma cell line secreting monoclonal antibody against prostate specific membrane antigen.

目的 制备具有良好器官特异性的抗前列腺特异膜抗原单克隆抗体。

The cross reactivity with other SAs was less than 1×10~(-3)%. The affinity constant of the anti-SQ monoclonal antibody SQ_(12)A_(10) was 1.40×10~9L/mol.

经鉴定，三株单抗与其他八种磺胺类药物交叉反应率＜1×10~(-3)％，其中SQ_(12)A_(10)单克隆抗体亲和常数为1.40×10~9L／mol，。

The relative cross-reactivity of PA with AFB-1,ZON,T-2,FB were less than 0.01%and with an affinity constant for PA of about 1.54×10~8 liters per mol.It offered a foundation for developing immunoassay method for PA.

单克隆抗体与黄曲霉毒素B1、玉米赤霉烯酮、T-2毒素和伏马毒素B1均无交叉反应率，单抗的亲和常数为1.54x10~8 L/mol，为进一步建立青霉酸的免疫学快速检测方法提供了基础。3。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

. Conclusions:(1) AA-Ⅰ could induce apoptosis of HK-2 and the secretion of FN and TGF-β1 at a range of concentrations. Analysis of their time course and the blocking test indicate that those effects of AA-Ⅰ are partly mediated by TGF-β1.(2) AL-Ⅰ as one metabolite of AA-Ⅰ could induce the direct injury of HK-2, which potency was higher than AA-Ⅰ.

(1)在一定浓度范围内AA-Ⅰ能够导致肾小管上皮细胞凋亡、并诱导FN和TGF-β1分泌，上述事件发生的时相关系分析及抗TGF-β1中和抗体阻断试验结果表明，AA-Ⅰ导致肾小管上皮细胞损伤的作用可能是由TGF-β1所介导的。

The variable region genes of the light and heavy ehains obtained from three atems of McAb against metal-bound tetrapeptide were joined into a single chain by a linker.

作者将获得的抗3株金属螯合匹肽单克隆抗体的轻、重链可变区基因，通过linker连接成单链基因，并在其后插入编码CGRP的N端前十三肽的39bp基因片段，组成Lv－linker－HvCGRP。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。