抗抗体

The monoclonal antibody produced by the hybridoma cell was teste...

结论制备出能分泌具有高特异性和亲和力的抗总黄曲霉毒素单克隆抗体的杂交瘤细胞株。

Further,with indirect immunohistochemistry technique,CD25(IL-2 receptor or IL-2R) cross-reaction has been identified on lymphocyte membrane of Ctenophaxyngodon idellus and Trionyx sinensis using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD25(IL-2R).

进一步用抗人白细胞分化抗原CD25(IL-2受体，简称IL-2R)单克隆抗体进行免疫组化交叉反应提示草鱼、中华鳖淋巴细胞膜上含有与人类IL-2R类似功能和结构的物质。关键词：白细胞介素-2；白细胞介素-2受体；发育和比较免疫学；草鱼；中华鳖

The rats in CpG and GpC groups were injected with CpG-ODN and GpC-ODN respectively every other day for 3 times, each at a dosage of 300 μg, and those in M group were uninjected.

注射抗－thy 1.1单克隆抗体后，隔天，CpG组经尾静脉注射CpG-ODN， 300μg/只，GpC组经尾静脉注射GpC-ODN，300μg/只，隔日注射，共3次。

We used the immunohistochemical ABC method to detect the expression of NF-κB at 2、 6、 12、 24、 48、 72 hours after infected by Salmonella typhimurium, and we deal the data statistically with univariant analysis of variance and Student-Newman-Keuls multiplerange test.

三组分别在感染鼠伤寒沙门氏菌后2、6、12、24、48、72小时6个时点，用免疫组织化学方法(抗NF-κB p65抗体、ABC法，石蜡包埋)和阳性细胞面积分布率半定量测定小肠、结肠及肾脏中NF-κB活性表达百分率，采用单变量多因素方差分析和Q检验做统计学处理。

Both the recombinant vaccina viruses expressing the mutated or wild E6 protein could induce the specific antibobies, but neither of them could produce CTL and immune protection.

表达野生型和突变型E6的重组痘苗病毒可诱发C57BL／6小鼠产生特异性抗体，但都未能诱发CTL反应，在抗肿瘤移植实验中也未显示出免疫保护作用。

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

Anticoccidial index increased by 73.50, rate of weight increased by 39.19%, mortality decreased by 33.4% in chickens inoculated with E. tenella by administering vitelline antibody.

tenella鸡的死亡率降低33.34%，增重率和抗球虫指数分别提高39.19%和73.50；(3)特异性高免卵黄抗体对E。

Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时，得到了60kD和40kD两种分子，用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定，在纯化过程中极易降解，40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的，而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

Immunoprecipitation reaction of three hemocyanin types was tested with antiserum against female specific protein and hemocyanin. It shows that the FSP and hexamer have the higher cross reaction thus they may have similar molecular structure.

利用抗FSP血清对三种型式血蓝素做扩散免疫沈淀试验，显示FSP与六隅体血蓝素有部份相似可被抗体辨识的部位，推测FSP与六隅体血蓝素的分子结构比较相近。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。