抗抗体

Most patients who have rheumatoid arthritis have a positive test for rheumatoid factor and anticyclic citrullinated peptide antibodies, as well as an elevated erythrocyte sedimentation rate and C-reactive protein.

大多数类风湿关节炎患者要进行类风湿因子、抗环瓜氨酸肽抗体阳性测定及红细胞沉降速率及 C 反应蛋白增高的检查。

Our aim was to compare the practical usefulness of magnetic resonance imaging of the hand versus anticyclic citrullinated peptide antibody testing to confirm the diagnosis of clinically suspected RA in the absence of rheumatoid factor and radiographic erosions.

本研究比较手核磁共振与抗CCP抗体对诊断无类风湿因子和放射学糜烂、临床怀疑类风湿关节炎患者的实际有用性。

AIM: To realize an antiricin neutralizing monoclonal antibodybased ScFv proteins with high biological activity in the pCDNA3.1 vector expression system and to evaluate the neutralizing activities of ScFv.

目的：实现基于中和性单抗4C13单链抗体的真核表达，并对其生物学活性进行评价。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

An ELISA was developed for detecting antibody against Aspergillus fumigatus and would be useful in early diagnosis of IA.

建立了检测抗烟曲霉分泌蛋白抗体的ELISA法，在IA早期诊断中具有潜在应用价值。

The role of LM609 in bone marrow micrometastasis in athymic mice was detected by quantitative real-time RT-PCR.

实时荧光定量RT-PCR检测抗整合素αvβ3抗体LM609对裸鼠乳腺癌骨髓微转移模型的影响。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

On day 3 post-booster-immunization with the bacterially expressed gC protein MBP-gC-NC,the myeloma cell line SP2/0 was fused by PEG4000 with the splenocyte of mice after booster immunization.Then three McAbs,1B4,1H4 and 2C8,were obtained and characterized with titers of 1∶2×105-1∶2×106 in ascites.

给BALB/c小鼠用大肠杆菌表达的gC融合蛋白MBP-gC-NC加强免疫后第3d，取小鼠脾细胞与SP2/0小鼠骨髓瘤细胞在PEG4000作用下进行杂交融合，经筛选、克隆，获得了3株稳定分泌抗PRV gC蛋白单克隆抗体的杂交瘤细胞株，分别命名为1B4、1H4和2C8。

Methods With indirect immunofluorescence assay and enzyme-linked immunosorbent assay, an investigation on antibodies of Borrelia burgdorferi was carried out in sera of 827 suspected Lyme disease patients.

方法2001-2006年应用间接免疫荧光试验和ELISA 2种方法对来自全国各地的827例临床拟诊莱姆病患者血清进行抗伯氏疏螺旋体IgM、IgG抗体检查。

The results showed that 10(6.5%) residents had positive IgG titers (1∶128) against Borrelia burgdorferi 6(3.9%) residents were patients with clinical Lyme disease.

结果：血清学检测155人林区居民，抗莱姆病螺旋体IgG抗体阳性10人，自然感染率6.5%；其中经临床和血清学确诊莱姆病患者6例，患病率3.9%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。