抗抗体

By adding a sequence encoding a small peptide,〓, to the base sequence of pYZFcd20, a new phasmid pYZcpp3 was constructed. Using the pYZcpp3 to transform E. coli. 16c9, the genetically engineered bacteria 10916# was obtained. 10916# can secret the soluble chimeric antibody fragment anti-CD20 F〓 into periplasmic.

通过对质粒pYZFcd20的碱基序列的改造，在CH1基因的末端添加了一截二聚化结构的编码序列，实现了在大肠杆菌中直接表达可溶性抗CD20嵌和抗体片段F〓的目的。

Phycoerythrin labeled anti-human CD45 monoclonal antibody and flow cytometer were purchased from BD.

藻红蛋白标记的抗人CD45单克隆抗体与流式细胞仪均为BD公司产品。

At 3 months after birthing, blood was obtained via the caudal vein. In the dark, phycoerythrin labeled anti-human CD45 monoclonal fluorescent antibody was added. Flow cytometry was used to measure CD45 expression. Rebuilding effect of human blood cells in mice in vivo was detected by positive rate.

待小鼠出生后3个月，经尾静脉取血，在避光状态下加入藻红蛋白标记的抗人CD45单克隆荧光抗体，流式细胞仪检测血细胞表面标记CD45的表达，通过其阳性率判断人血液细胞在小鼠体内的重建效果。

Nine curses together with death befell Eve in consequence of her disobedience Pire R.

9诅咒一起死亡降临前夕的后果，她抗命（ pire传译下午第十四；抗体。

Diagnostic usefulness of a third-generation anti-cyclic citrulline antibody test in patients with recent-onset polyarthritis.

现代预防医学， 2007， 34(2)： 346.20 石宏宴。抗CCP抗体与RF联合检测在类风湿关节炎中的临床意义。

Results:The sera of 500 forest residents were tested for antibodies against Borrelia burgdorferi(strain B31),32(6.40%) residents had significant IgG titers.38 cases,including Erythema Chronicum Migrans,arthritis,facial paralysis,polyneuritis,mengitis and cardiac abnormalities,were confirmed being Lyme disease by serological and clinical examination.

结果：共查山林地区居民500人，血清抗莱姆病螺旋体抗体阳性者32人，阳性率6.40%。38例临床和血清学确诊的莱姆病患者，其主要的临床表现有环形或慢性游走性红斑、关节炎、面神经麻痹、多发性神经炎、脑膜炎和心脏损害等。

Using Gαq/11 polyclonal antibody as prole and immunoblot analysis, we measured Gαq/11 contents in left ventricle of rats. There was a significant decrease in Gαq/11 content of CA rats compared with values of control animals after 3 days of operation (P＜0.01). Gαq/11 contents were not modified in left ventricle after 10 and 30 days of stenosis.

以兔抗鼠Gαq/11多克隆抗体为探针，用免疫印迹方法测定了大鼠左心室组织中Gαq/11蛋白的相对含量，结果发现术后3天CA组大鼠左室中Gαq/11含量较SO组明显降低(P＜0.01)，术后10天和30天二组大鼠心肌中Gαq/11含量无差异(P＞0.05)。

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性，进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠，3次免疫小鼠后，血清(1：1000)中能检测到较强的MUC1抗体阳性；以最大OD450值之纯化血清做为一抗行蛋白印迹分析，结果显示：纯化血清可识别MUC1蛋白；小鼠脾脏淋巴细胞增殖率检测结果表明：噬菌体展示肽表位可在无佐剂存在情况下，大大促进小鼠的脾淋巴细胞的增殖，证明噬菌体是一较好的生物载体。

A sandwich LAB-ELISA wa s developed to detect Pseudorabies virus on the basis of species-specific monoclonal antibodies in this study.

在伪狂犬病病毒种特异性单克隆抗体的基础上建立了检测该病毒抗原的单抗夹心LAB-ELISA方法。

Based on the rabbit-anti-PRV IgG and McAb, the double sandwich enzyme-linked immunosorbent assay for detection of Pseudorabies virus was developed.

利用所制备的兔抗PRV IgG和McAb建立了检测PRV的"单克隆抗体双夹心ELISA"。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。