抗抗体

Objective To explore the clinical significances of ACA,ANA and ENA of patients with chronic idiopathic thrombocytopenic purpura.

目的 探讨慢性特发性血小板减少性紫癜患者抗心磷脂抗体、抗核抗体和抗可提取性核抗原抗体检测的临床意义。

We coupled the IgG of monoclonal antibody N34 specific to UK and the Fab of monoclonal antibody SZ-51 specific to the membrane protein GMP-140 of activated platelet. The bispecific antibody (N34 SZ-51) is able to bind UK and plasma-clot simutaneously. It enhanced plasma clot lysis 5 fold by HUK whereas it enhanced plasma clot lysis 38 times by LUK. UK in association with the bispecific antibody didn't decrease the concentration of fibrinogen in the assay supernatants.

利用抗体介导使纤溶酶原激活剂定向到血栓表面，可大大提高其纤溶作用，我们用双功能团试剂将抗尿激酶的单克隆抗体N34的IgG和抗活化血小板膜蛋白GMP-l40的单抗SZ-5l的Fab片段通过二硫键共价偶联，合成的双专一性抗体（N34-SZ-51）既具有血栓亲和性也具有尿激酶亲和性，从而增加了尿激酶对血栓的专一性，使高分子量尿激酶溶栓率提高了5倍，使低分子量尿激酶溶栓率提高了38倍之多，且不影响血浆中纤维蛋白原的含量。

Results: 36 cases with 22 cases of MSAs and MAAs positive (61%), which MSAs positive rate was 40 percent, the most common MSAs was the anti-Jo-1 antibody (30%), MAAs the positive rate was 28%. Anti-Ro-52 antibody for 18 per cent, and with the anti-Jo-1 antibody highly relevant.

结果：36例患者中22例出现MSAs和MAAs阳性(61%)，其中MSAs阳性率为40%，最常见的MSAs是抗Jo-1抗体(30%)，MAAs的阳性率为28%，抗Ro-52抗体占18%，并且与抗Jo-1抗体高度相关。

Selection criteria: Hypothyroidism with the increased thyroid microsomal antibodies, thyroid peroxides antibody, or thyroglobalin antibody which detected in city, and provincial hospital in gestation or before pregnancy were diagnosis as Hashimotos thyroiditis. Hyperthyroidism supported with the thyroid ultrasonography or ECT scans or confirmed by fine-needle-aspirate biopsy of the thyroid, excluded the hyperfunctioning thyroid adenoma and multinodular goiter, were diagnosis as Graves disease.

纳入标准：对妊娠前或妊娠期间曾在省、市级医院检查有甲状腺功能减退且伴有抗微粒体抗体或抗甲状腺过氧化物酶抗体、抗甲状腺球蛋白抗体增高者为慢性淋巴细胞性甲状腺炎孕母；有甲状腺功能亢进且甲状腺B超或甲状腺ECT扫描或甲状腺细针穿刺病理检查证实、并排除高功能腺瘤及多发性结节者为GraveS病孕母。

Methods: By using JSB-1 with intramembranous antigen determinant as antibody, the positive rate as optimization index and MCF-7/A as model cells, cell pretreatment methods, such as the presence or Absence of FACS permeabilizing solution, the concentration of FACS permeabilizing solution, the time treating cells with FACS permeabilizing solution, the time incubating cells with JSB-1 working fluid, the time incubating cells with goat-anti-mouse IgG-FITC solution, and the conditions for keeping JSB-1 working fluid and cell numbers, were optimized.

采用抗原决定簇在细胞膜内的单抗JSB-1，以阳性百分比为优化指标，耐阿霉素人乳腺癌细胞系MCF-7/A为模型细胞，对FACS透化溶液的有无、FACS透化溶液的浓度、FACS透化溶液处理细胞的时间、鼠抗JSB-1抗体的孵育时间、羊抗鼠IgG-FITC抗体的孵育时间、JSB-1抗体工作液的保存条件、以及细胞数量进行优化。

To develop standard WNV diagnostic tools, four monoclonal antibodies against WNV envelope protein domain III were produced. The isotype, the reactivity with denatured and native antigens, the affinity and specificity of antibodies have been characterized. Two MAbs (4B3 and 2F5) were used to establish an antigen capture-ELISA detection system.

为了建立有效的WNV检测方法，本研究制备了四株抗WNV囊膜蛋白第三结构域的单克隆抗体，鉴定了抗体的亚型、抗体和变性及非变性抗原结合的强度、抗原和抗体结合的亲和力常数，进行了免疫荧光分析、测定了抗原表位竞争、抗体的中和能力等，并检测了上述单抗与日本乙型脑炎病毒的交叉反应情况。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原，注射新西兰白兔制备出效价高达1：32000的多克隆抗体作为第一抗体，第二抗体为辣根过氧化物酶标记的羊抗兔IgG，通过交叉稀释法确定抗原、一抗及二抗的最优反应条件，建立酶联免疫吸附分析方法体系，病毒检测灵敏度达30ng。

THE CORRELATION OF ANTITROPHOBLAST ANTIBODY, EMAB AND HCG-AB WITH IVF-ET PREGNANCY OUTCOMEYANG ZONG-ZHI, SONG DONG-PO, ZOU SHU-HUA, et al（Medical and Health Care Center For Women and Childen of Qingdao, Qingdao 266011, China）［ABSTRACT］ObjectiveTo assess the effect of antitrophoblast antibody, EmAb and HCG-Ab on in vitro fertilization and embryo transfer outcome.MethodsThis study included 140 infertility women of unexplained reasons, salpingemphraxis and/or male factor undergoing IVF-ET, of whom, 31 with repeated failure of implantation; 26 with biochemical loss of pregnancy; 18 with clinical pregnancy loss. Sixty-five with clinical pregnancy served as control group.

目的 探讨抗滋养细胞膜抗体、抗子宫内膜抗体、抗HCG抗体对体外受精-胚胎移植妊娠结局的影响方法选择2002年1月～2007年7月在本生殖医学中心由于不明原因性不育、女方输卵管因素和男方轻度少弱精子症因素不育而接受IVF-ET治疗的妇女140例，其中次植失败31例，生化妊娠丢失26例及临床妊娠丢失18例，持续妊娠65例。

Methods Phosphotyrosine was first combined with hemocyanin from keyhole limpets. Then, rabbits were immuned with the complete antigen and antisera were gained. The ELISA system consisted of purified antiphosphotyrosine antibody as first antibody and sheep antirabbit lgG-HRP as second antibody. Results CV within the batch was 8.4%.

应用合成的磷酸酪氨酸-钥孔帽贝血蓝蛋白免疫家兔得到抗血清，将纯化的抗磷酸酪氨酸抗体作为第一抗体，羊抗兔Ig酶结合物作为第二抗体，建立双抗体夹心酶联免疫吸附测定法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。