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Methods: fifteen sd rats were randomly divided into five groups (n=3), and adrenalectomy was performed and anterior pituitary specimens were obtained after a week. anti gap 43 antibody and anti adrenocorticotrophic hormone, thyroid stimulating hormone, luteinizing hormone, prolactin and growth hormone antibodies were used in the double immunohistochemical staining.

将成年雄性sd大鼠15只随机分为5组(n=3),手术切除大鼠双侧肾上腺,1 wk后取垂体前叶标本分别进行抗gap 43与抗促肾上腺皮质激素、促甲状腺激素、黄体生成素、催乳素、生长激素抗体的免疫组织化学双标染色,观察gap 43 li神经纤维与各腺细胞之间的关系。

Methods: Seventy-eight specimens of disc herniation(classified as protrusions,, extrusions, and sequestrations) and fourteen nonherniated discs were immunostained with antihuman MMP-3 monoclonal antibody, using the avidin-biotin-peroxidase complex method.

运用免疫组织化学抗生物素蛋白—生物素—过氧化物酶复合体法,通过抗MMP3的单克隆抗体,对78例椎间盘突出症和14例非椎间盘突出症的椎间盘标本进行染色。

To analyse the reason for one case of hemolytic transfusion reaction, antibodies in a patient's serum were identified using panel cells and Le phenotype cells, patient phenotype was identified by using anti-Le and anti-Le blood grouping reagents and the entire coding region of FUT3 gene was amplified by PCR and sequenced directly.

为了分析1例溶血性输血反应产生的原因,用谱细胞和Le表型细胞鉴定患者血清中的抗体,用抗Le和抗Le血型试剂鉴定患者红细胞表型,用PCR测序方法分析LE基因(FUT3基因)全编码区序列。

Methods AGOs polypeptide was synthesized by chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen. Then AGO-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性AGO多肽,以马来酰胺活化匙孔血蓝蛋白(keyhole limpet hemocyanin, KLH)作为载体构建多肽免疫原,通过致敏大白兔,制备系列兔抗人AGO多克隆抗体,然后用亲和层析法纯化抗体。

Competitive inhibition curve was obtained in the presence of unlabelled oncoM.

放射免疫测定表明,当抗血清稀释度为1:4000时,125I-oncoM与特异性抗体结合率达40%,与非标记抗原能竞争结合抗体,获得良好的竞争抑制曲线。

To detect the distribution conditions of SV5 antibody in laboratorial simians, two antibody detection methods, IFA and IEA, were established using the simian SV5-positive serum provided by Lab Animal Research Institute of China Academy of Medical Science.

为检测地鼠群中SV5抗体分布群况,我们利用细胞培养的方法制备了SV5抗原并免疫SPF地鼠获取了地鼠抗SV5阳性血清,建立了针对地鼠血清抗体的IEA和IFA两种检测方法。

We made all-round serological tests to the two RhD-- individuals,including identification of blood group、Coombs test、antibody screening and identification、antibody titer and HDN test,together with investigation of genealogical blood group to all family members by classic hemagglutination methods.

我们采用经典的血细胞凝集法,对2个体进行了血型血清学检测,包括血型鉴定、抗人球蛋白试验、抗体筛选与鉴定、抗体效价检测与新生儿溶血病检测,并对两个家系所有家系成员进行家系血型调查。

Currently, the anti-influenza antibody is measured by the hemagglutinin- inhibition test. Although it is simple, but the result is easily influenced by the quality and species source of the red blood cells used. Antibody titer determined by HI test cannot reflect the protection titer either.

血球凝集抑制试验(hemagglutinin- inhibition test,HI test)是一至今仍普遍使用来测定抗流行性感冒病毒抗体的方法,此方法虽然快速、简便,但测定结果容易因为红血球的品质及来源而受影响,且血球凝集抑制试验所测定出的抗体效价亦不能反应真正的保护能力。

"Rh-negative persons who receive Rh-positive Blood transfusions produce antibodies to Rh factor , which attack red Blood cells with the factor if they are ever received again, causing serious illness and sometimes death."

Rh阴性者接受Rh阳性血液输血时会产生抗Rh抗体,之后若再输入Rh阳性血液,则抗体将攻击这些Rh阳性的红血球,引起严重的症状,有时甚至死亡。

AIM: To construct the transgenic Arabidopsis thaliana containing fulllength gene of mouse/human chimeric antibody(3G1MH) against Hantaan virus.

目的:建立能表达抗汉滩病毒中和性鼠/人嵌合抗体基因的转基因植株,为汉坦病毒基因工程抗体的研究提供实验基础。

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这一模式非常关注商人的网络信用基础。

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