抗抗体

It is highly valuable to diagnose seronegative and early RA.

AFA 抗体与抗CCP 抗体联合检测，灵敏度89.50%，特异度达98.71%。

Furthermore, the study of quantitative protein chip found that rat antibodies against hPolyHb, bPolyHb or pPolyHb only slighty or even did not cross-react with the Hb/PolyHb of other two species.

此外，抗原芯片方法定量检测结果还表明由大鼠产生的相应抗人，牛或猪血红蛋白的抗体大多不产生或产生很弱的抗原抗体交叉结合反应。

Notably,we also found that anti-CTLA4 can wellsynergize with B7-expressing tumor vaccines.This result is different with that ofHurwitz et al obtained from a similar research in 1998,in which they found thatanti-CTLA4 can not synergize with B7-expressing tumor vaccines.

值得注意的是，在我们的实验中发现anti-muCTLA4抗体与B7瘤苗联合治疗小鼠肿瘤具有很好的协同效应，这与1998年Hurwitz等在类似的研究中所得出的结论不同，即他们认为anti-muCTLA4抗体与B7瘤苗间没有协同抗小鼠肿瘤作用。

Results:Hybridoma cell lines excreting monoclonal antibodies were developed and monoclonal antibodies against FB1 were prepared.

结果：建立了稳定分泌抗FB1毒素单克隆抗体的杂交瘤细胞株，分泌的抗体为IgG1亚类，分子量150KD，腹水稀释度为1：2.0×108，亲和常数为6.72×109 L/mol。

The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice. In addition, the blocking duration of anti PI-IgG is associated with antibody concentration.

纯化的抗淋球菌GST-PI融合蛋白的多克隆抗体，能有效抑制淋病奈瑟菌对小鼠泌尿生殖道上皮的黏附与感染，阻断作用及持续时间与抗体浓度有关。

Polyclonal antibodies for Ochratoxin A were generated from rabbits after the animals had been immunized with either OTA -γ- globulin or OTA - keyhole limpet hemocyanin. A competitive indirect enzyme - linked immunosorbent assay and a competitive direct ELISA were used for the characterization of the antibodies and for analysis of OTA in coffee, corn, barley and bean samples.

本实验利用OTA -γ- globulin和OTA - KLH两种不同的OTA接合物为抗原来免疫兔子，进而收集免疫血清以获得抗OTA 的多株抗体，接著利用此一抗体来建立非直接型竞争酵素连结免疫吸附分析法和直接竞争型酵素连结免疫吸附分析法，并用cdELISA的方法加以侦测咖啡及玉米类、麦类、豆类样品中赭麴毒素A 的含量。

Draining lymph nodes isolated from the BALB/C mice immunized with rabbit skin collagen Type Ⅲ accord- ing to a rapid immunization protocol, the lympho- cytes were fused with the myeloma cell line NS-1, 3 out of total of 201 growing hybridomas we obtained were producing collagen Type Ⅲ reactive antibodies after 3 limited dilution clones performed.

本实验采用快速免疫的方法用兔皮肤Ⅲ型胶原免疫BLAB/C小鼠，取淋巴结的细胞与小鼠骨髓瘤细胞NS-1融合，240孔中201孔有杂交瘤生长，用ELISA检测有6孔反应阳性。选3孔生长良好，抗体浓度高的细胞进行有效的有限稀释法克隆3次，获3株分泌抗Ⅲ型胶原单克隆抗体的杂交瘤细胞。其中二株属IgG2b，一株为IgG1。

It was suggested that isotypes of human xenoreative natural antibodies against porcine pancrease islet cells were IgG and IgM.

结果表明：人抗猪胰岛细胞天然抗体的同种型是IgG和IgM型抗体。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原，免疫BALB/c小鼠，按常规方法进行融合，间接ELISA方法进行筛选，采用有限稀释法，经过三次克隆，最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5)，分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类，三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。