抗抗体

Methods BALB/c mice were immunized by cytoderm antigen of Aspergillus fumigatus, excreting antigen and inactivated conidiospore respectively, to prepare monoclonal antibodies specific to Aspergillus. Cross-reactions between mAbs and antigens from Aspergillus or Monilia genus were characterized by immunofluorescence.

采用烟曲霉细胞壁抗原、分泌抗原和灭活分生孢子，分别免疫BALB/c小鼠，制备特异性的抗曲霉单克隆抗体，免疫荧光法鉴定单克隆抗体与曲霉属和念珠属抗原的交叉反应。

For developing an immunoassay for multiple residues of pyrethroid pesticides, the hapten N-2- carbanmoyl-(3"-phenoxyphenyl) methyl 3-(2"-Cldoro-3",3",3"-trifluoropropenyl)-2, 2-dimethylcyclopropane- carboxylate was synthesized from cyanohydrin and 3-(2"-Chloro-3",3",3- trifluoropropenyl)-2, 2-dimethylcyclopropanecarboxylic acid via hydrolysis and esterification. The synthesis of the hapten was foundation of preparaing anti- cyhalothrin polyclonal antibodies.

为了制备对单一拟除虫菊酯高特异性的抗体，以菊酸和氰醇为起始原料，通过水解、酯化反应合成了半抗原N-2-氨甲酰-(3-苯氧基苄基)甲基-3-(2-氯-3，3，3-三氟丙烯基)-2，2-二甲基环丙烷羧酸酯，为进一步制备抗三氟氰菊酯的特异性抗体奠定基础。

Based on hemagglutination specificity test,absorption and eluate test,and hemagglutination inhibition assay,it was showed to be specific only for the human H blood ...

杂交瘤细胞在液氮中贮存1年，分泌抗体能力不变。抗体经56℃保温30min及-20℃和37℃反复冻融试验，凝血活性保持稳定。可望能取代传统的抗H试剂而用于临床血型分型。

Methods PIWIL4 peptide was synthesized using chemical method, and conjugated to Keyhole limpet hemocyanin as immunogen.

方法合成特异性PIWIIA多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIIA多克隆抗体，然后用亲和层析法纯化抗体。

The number of chickens in each group is 16 for SPF chicken At 4 weeks of age, chickens were eye-drop immunized with the recommended dose of commercial IBV vaccine, or vaccinated intradermally via wing with 5×106 PFU rFPV-IBVS1 or 5×106 PFU S-FPV-017. Four weeks after vaccination, chickens in all groups were challenged by bronchitis injection with a lethal dose (0.2ml 8.5EID50/0.1ml) of IBV XJ3 strain After challenge, chickens immunized with rFPV-IBVS1 and commercial IBV vaccine were all protected from death; the result indicated that rFPV-IBVS1 can protect chickens from the infection of IBV.

攻毒后的结果显示所有含rFPV-IBVS1免疫组的SPF鸡均100%保护而免于死亡；IBV弱毒疫苗免疫的SPF鸡也获100%保护，免疫以后的抗体跟踪检测证明rFPV-IBVS1诱导的抗IBV抗体水平低于IBV弱毒疫苗免疫组；攻毒后进行的IBV分离和PCR检测结果表明IBV弱毒疫苗免疫的SPF鸡能够更好地阻止攻击强毒的感染。

The model had a high incidence and its pathologic changes were close to those of multiple sclerosis, so it might be an ideal model for the research of MS. 2. It is supposed that the activity of T cell response to MOG_(35-55) and the increases of anti-MOG_(35-55) antibody and IFN-γlevels may play key roles in the onset of EAE.

通过比较EAE小鼠和正常小鼠T淋巴细胞的增殖、血清抗MOG_(35-55)抗体和IFN-γ水平的不同，显示T细胞的活化、抗体的升高、细胞因子的异常可能共同导致了EAE的发生。

BALB/c mice were co-inoculated muscularly by this plasmid and the nucleic acid vaccine plasmid pVAXGE expressing the HIV-1 (Human immunodeficiency virus type I) gag-gp120 protein. The level of serum antibodies after immunization showed that the specific antibodies against HIV-1 appeared at the second week and raised to the peak level at the sixth week for the co-immunization group.

将它与表达I型人免疫缺陷病毒(Human immunodeficiency virus 1， HIV-1) gag-gp120的核酸疫苗质粒pVAXGE共同肌肉注射BALB/c小鼠，免疫3次后，以ELISA法检测免疫小鼠血清中抗HIV-1抗体水平，结果显示联合免疫组小鼠在免疫2周后已有抗体产生，6周后进入高峰。

For recombinant nucleoprotein and spike protein,we developed an ELISA to test these two antibodies.

以ELISA法分别检测抗-SARS-CoVIgM和IgG抗体、SARS-CoVN蛋白和S蛋白IgG抗体。

Department of Periodontology,School of Stomatology,Beijing Medical University,Beijing 100081,China

但抗体水平与临床指标的关系结论不一，抗Pg抗体具有保护作用还是破坏作用仍需探讨。

AIM To observe the expression of the selected antibodies from the constructed phage display library.

目的 观察从已构建的人抗-HBsAg噬菌体抗体库中筛选出的特异性抗体的表达情况。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。