抗抗体

Final diagnoses at 1-year follow-up were RA in 31 patients, undifferentiated arthritis in 7 (5 self-limiting), and psoriatic arthropathy and antisynthetase syndrome in 1 patient each.

仅7例抗CCP抗体阳性，但最后均诊为类风湿关节炎，在该组血清阴性类风湿患者中的抗CCP阳性率仅23%(7/31)，其诊断特异性达100%(95%可信区间为71.7-100)，敏感性为23%(95%可信区间为9.6-41.1)，而用MRI标准则100%的类风湿患者诊断正确，另外有2例假阳性结果(1例银屑病关节炎和1例抗合成酶综合症)，MRI标准对诊断血清阴性类风湿关节炎的特异性为78%(95%可信区间为40.0-97.2)，敏感性为100%(95%可信区间为90.8-100)。

Now, CD11a monoclonal antibody, LFA-3/IgG1 fusion protein and TNF-αhave been successfully applied in the immunotherapy of psoriasis, and animal experiments have primarily confirmed the therapeutic effects of anti-granulocyte-macrophage colony stimulating factor IgG1,anti-CD4 monoclonal antibody and anti-complement receptor 3 antibody, which are hopeful to be the breakthrough of psoriasis treatment in the future.

一些新的免疫学研究发现，CD11a单抗、LFA-3/IgG1融合蛋白、TNF-α已经成为银屑病免疫治疗中较为成熟的疗法，而粒细胞-巨噬细胞集落刺激因子单抗IgG1，CD4单抗及补体受体3抗体的治疗作用在动物实验中已得到初步验证，有望为银屑病的治疗提供新的突破点。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

In this study,an indirect immunoperoxidase technique based on hyperimmune serum was developed to detect avian nephropathogenic infectious bronchitis virus in paraffin_embleded tissue sections of immune organs.

以鼠抗鸡肾型传染性支气管炎病毒的高免血清为一抗，辣根过氧化物酶标记的羊抗鼠IgG为二抗，建立了检测石蜡切片肾型IBV抗原的间接酶标抗体染色技术。

Sera were collected from 119 parous cows. After screening and testing these sera with cell panel, a total of 6 kind reagents against BoLA A2, A6, A8/14, A10, A11 and A30 were typed from 16 alloantisera. The titer of antisera ranged from 2x to 8x. Besides these 16 sera, another serum which seemed to be against subtype of A30 was also obtained.

第一种方法系采集经产母牛之血清经比对测试后，筛选出具特异性抗体之血清；以此方法共筛选 119 头经产母牛之血清，而制得 6 种标准抗血清计 16 支，分别对抗 BoLA A2、A6、A8/14、A10、A11、A30 等抗原型，此外尚制得 1 支为疑似对抗 A30 之亚型之抗血清；所得抗体力价偏低，介於 2～8 倍间。

In our work to select high affinity antibodies from reshaped phage antibody library against human bladder carcinoma, we found a panel of antibodies with much different antigen binding activity in library screening had similar relative affinity constants.

从抗人膀胱癌改形噬菌体抗体库中筛选出一组相对亲和力常数和细胞结合活性相近的单链抗体，而它们的结合活性均明显高于起始的鼠源单链抗体。

And 98. 3% respectively Conclusions To all the eleven antinuclear autoantibodies , the sensitivity is all above 95.0% and specificity is all above 98.0%, which indicate that there is high concordances between the ANAs protein chip and the methods used in clinical screening and confirmation, and it could meet the requirement of clinical autoantibodies profile detection.

结论蛋白芯片技术检测11种抗核抗体谱自身抗体的检测敏感度（均〉95.0％）和特异度（均〉98.0％）均较高，且与IIF和EHSA法有较高的符合率，能满足临床检测自身抗体谱的要求。

Objective: To observe production of antibody to rhbFGF, its neutralizing activity and deposition in kidney in conducting toxicity study on repeated doses of recombinant human basic fibroblast growth factor in normal rhesus monkeys and Wistar rats.

目的：在猕猴和大鼠中进行重组人碱性成纤维细胞生长因子的重复给药毒性试验时，观察给药后抗 rhbFGF 抗体的产生和抗体的中和性，以及抗体在肾脏的沉积。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。