抗抗体

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

These experiments show that phycoerythrin conjugates are a valuable class of fluorescent reagents for analysis of cell surface antigens.phycoerythrin conjugates can be used together with fluorescence-labeled reagents for two-color analysis.

结果 用R-PE标记的抗CD 3 、抗CD 4 、抗CD 8 抗CD 19 、抗CD 45 等单抗试剂，检测正常人外周血白细胞表面相应抗原的表达，流式细胞仪分析表明，R-PE标记的抗体特异性保持完好，且荧光强度高，还可配伍成双标试剂。

The purpose of this research is to generateengineering antibody by using phage display technology,and to investigate its binding activitywith the pseudorabies virus.

本研究的目的就是克隆抗伪狂犬病病毒抗体重链、轻链可变区基因，构建单链抗体基因，利用噬菌体展示技术制备噬菌体抗体，并在大肠杆菌中进行可溶性表达，获得具有对伪狂犬病病毒结合活性的表达产物，为以后在治疗、预防上的应用奠定基础。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

This investigation is designed to evaluate the value in comment on the treatment effects of the diagnostic kit made by monoclonal anti idiotypic antibody NP30 of Schistosom japonicum .

为证实用日本血吸虫单克隆抗独特型抗体NP30制备的抗体检测试剂盒考核治疗效果的价值，采用双&抗原&夹心酶联免疫吸附试验，用NP30替代抗原，检测血吸虫病抗体。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示，抗MOG和抗MBP抗体可以预测更多和更早期的发作，39名血清呈阴性的患者中，9位(23%)复发，而重新发作的时间是45 ± 3.7个月，22名带有抗MOG和抗MBP的患者中，21名(95%)复发，复发时间为 7.5 ± 4.4个月，另外42名患者只有抗MOG，35人(83%)在 14.6 ± 9.6个月之内复发（P 。001，二组相较于血清呈阴性的患者）。

Anti-MOG and anti-MBP antibodies predicted more frequent and earlier relapses. Of 39 seronegative patients, nine (23%) had a relapse, and time to relapse was 45 13.7 months. Of 22 patients with anti-MOG and anti-MBP, 21 (95%) relapsed, and mean time to relapse was 7.5 4.4 months. Of 42 patients with only anti-MOG, 35 (83%) relapsed within 14.6 9.6 months P .001 for both comparisons with antibody-seronegative patients.

研究显示，抗MOG和抗MBP抗体可以预测更多和更早期的发作，39名血清呈阴性的患者中，9位(23%)复发，而重新发作的时间是45 ± 3.7个月，22名带有抗MOG和抗MBP的患者中，21名(95%)复发，复发时间为 7.5 ± 4.4个月，另外42名患者只有抗MOG，35人(83%)在 14.6 ± 9.6个月之内复发（P 。001，二组相较於血清呈阴性的患者）。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

female Lewis rats with 6-8 weeks of age and 160-180g of weight were immunized subcutaneously into both hind footpads with 50μg Torpedo AChR emulsified in complete Freud's adjuvant in a total volume of 200μl and divided into 4 groups at random.

ELISA法动态观察外周血抗AChR抗体IgG含量以及特异性抗体亲和力的改变。3.ELISA法测定致敏后第45日，大鼠血清中AChR特异性IgG抗体亚型IgG1、IgG2a、IgG2b含量的改变。4。

The objective was to develop a rapid and simple colloidal gold immunochromatographic assay for the diagnostic of chicken NDV. Tri-sodium citrate with aqueous gold chloride were warmed up and mixed to make gold sol. Colloidal golds were coupled with the purified anti-ND antibody.

为了建立一种快速、简便的检测鸡新城疫病毒的胶体金免疫层析法，采用柠檬酸钠还原法制备胶体金颗粒，标记纯化的ND抗体，并包被在玻璃纤维素膜上，另外将纯化的ND抗体和纯化的兔抗鸡抗体包被在硝酸纤维素膜上，组装成ND快速检测试纸条。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。