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The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体,然后导入毕赤酵母(P8chia nastoris)菌株GSlls细胞中,在甲醇的诱导下,经过酵母高密度发酵进行PAP的表达,经SDS-PAGE分析,结果表明,在培养基上清液中含有一明显的特异性蛋臼条带,大小为34Ku,经Western-blotting分析,该蛋白与法国PAP抗血清有特异性反应,体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性,说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

Globulus oil has the anti-inflammatory effect on chronic bronchitis induced by lipopolysaccharide in rats and the inhibitio effect on hypersecretion of airway mucins.

蓝桉油对脂多糖引起的大鼠慢性支气管炎具有一定的抗炎作用,并能抑制其气道黏蛋白高分泌现象。

Antitumor effect of EGCG was determined using the xenografts models of human gastric carcinoma cell SGC-7901 in nude mice.

目的 观察表没食子儿茶素没食子酸酯对体外培养人胃癌细胞系(SGC-7901)细胞的增殖抑制作用和体内抗人胃癌作用。

This study was to observe the inhibitory effect of 5-Aza-CdR on the growth of human nasopharyngeal carcinoma cells and xenografts in nude mice, explore the possible mechanisms, and search for new treatment target of NPC.

本文观察了5-杂氮-2'-脱氧胞苷对人鼻咽癌细胞及其裸鼠移植瘤的抑制作用,探讨其抗肿瘤效应及可能的机理,寻找鼻咽癌治疗的新靶点。

Competitive inhibition curve was obtained in the presence of unlabelled oncoM.

放射免疫测定表明,当抗血清稀释度为1:4000时,125I-oncoM与特异性抗体结合率达40%,与非标记抗原能竞争结合抗体,获得良好的竞争抑制曲线。

Pathologic observation also proved these results .It was suggested that TWP and anti-DC McAb had immunosuppressive effect on AIT.

本文在建立小鼠AIT的基础上,观察雷公藤多甙与DC单抗在抑制小鼠AIT发生中的作用,探讨二者对AIT治疗的可能性。

Objective: To investigate the possible mechanisms of nobiletin for anticancer by studying the inhibition effects of nobiletin on tubulin polymerization.

中文摘要:目的:研究川陈皮素对微管蛋白聚合的抑制作用,探讨其抗肿瘤的可能机制。

This study applied the low density lipoprotein system to evaluate the antioxidant activity of 14 yam tubers.

本研究以抑制低密度脂蛋白过氧化系统评估14种山药之抗氧化力。

The antiatherogenic effect of rosa roxburghii tratt juice is largely due to its ability to inhibit the formation of foamy cells induced by Ox-VLDL.

刺梨汁通过抑制Ox-VLDL的促泡沫细胞形成来实现其抗动脉硬化的功能。

Inhibition of cell migration, adhesion to ECM and invasion into ECM of tumor cells and regulation of homeostasis between MMPs and TIMPs to maintain ECM integrity may be the basic mechanism of inhibitive effect of Ber on invasion and metastasis of tumors.

抑制肿瘤细胞的运动能力、与细胞外基质的黏附、对细胞外基质侵袭,调节MMP2/TIMP2表达的平衡,从而维持细胞外基质的完整性,可能是小檗碱抗侵袭和转移作用的机制之一。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。