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The affinity constant of E1-McAb reacting with coated complete antigen was 8.2×10^8L/mol. Biotin-streptavidin complex was used for the determination of E1. The calibration curve of free E1 covered a range of 10~10 000 pg/mL.

以生物素化的羊抗鼠免疫球蛋白及辣根过氧化物酶标记的链亲和素为标记体系,通过竞争抑制的方式测定游离的雌酮,结果表明:雌酮在10~10000pg/mL内呈线性关系。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05~101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明:筛选出的2株杂交瘤细胞,单抗亚类均为IgG1型,其中4G1-B3株的ENR mAb间接ELISA效价为1:1.024×106,亲和常数为9×1010L/mol;竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L,与其他化合物的交叉反应率均小于0.01%,鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%,不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小,试剂盒保存期6个月以上。

ARL-1 gene can reduce the cytotoxicity of anti-cancer drug consisting of active aldehyde group and suppress apoptosis induced by anti-cancer drug consisting of active aldehyde group.

ARL-1基因能降低含有活性醛基的抗肿瘤药物的细胞毒性,和抑制其所诱导的细胞凋亡。

Tripterygium Wilfordii Hook is a Chinese herbal plant possessing ant...

雷公藤是一种中草药,具有抗炎及免疫抑制作用。

Based on Fenton reaction and the auto-oxidation of pyrogallol, the anti-radical activity of morinda officinalis how was studied by spectrophotometry. The morinda officinalis how has obvious scavenging effects on hydroxyl radical and superoxide anion radicalO2(superscript - with the efficiency related to concentration of morinda officinalis how.

基於Fenton反应和抑制邻苯三酚自氧化原理,分光光度法测定体系吸光度的变化研究巴戟天抗自由基活性,证明巴戟天对羟自由基及超氧阴离子自由基O2(上标-均有良好的清除作用,清除率与巴戟天的浓度有一定的量效关系。

The microprocessor protection device is under EFT/B immunity test.Improved distribution power supply,ferrite core,EMI filter and transient voltage suppressor can be used in the microprocessor protection device to control EFT/B through experiments.

通过给微机保护装置的外部端口进行电快速瞬变脉冲群抗扰度试验,提出了改进的分布式电源、串联铁氧体磁珠、电磁干扰滤波器和并联瞬态抑制二极管等具体措施。

The inhibitory effect was strongest in King B2 medium, the optimum temperature for the highest antimicrobial activity was 33℃, and the optimal pH9.0. In the antagonistic test of BEB2 to the banana wilt disease, the inhibition was obvious with different in vitro methods, and the control effect of pot experiment was good.

结果发现,香蕉内生拮抗细菌BEB2为枯草芽孢杆菌;不同温度、pH值和培养基下的BEB2发酵液对香蕉枯萎病菌生长的抑制能力具显著差异,在King B2培养基上抑菌作用最强,最适抑菌温度为32 ℃,最适抑菌pH值为9.0。

An introduction is made on the application of an air supply low pressure jet aeration system in an improved oxidation ditch in the treatment of the liner board effluent with a detailed introduction and analysis on the commissioning of the operational process.

介绍了采用供气式低压射流曝气系统的改良型氧化沟用于箱纸板废水处理,并对整个调试运行过程进行了分析。改良型氧化沟采用全好氧曝气,沟中不存在缺氧区和厌氧区,抑制了传统氧化沟中的硝化与反硝化作用,降低了营养盐的投加成本,具有很强的抗冲击能力。

In the field of long-term antifertility, castor bean extract is of good effect which decreased mice's production and generations within 210 d (mice's normal gestation period is 21~23d).

蓖麻油长期抗鼠生育效果明显,在210d(正常小鼠的妊娠期是21~23d)内有效降低小鼠生育代数与产仔数,生育抑制率达80%以上。

Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2(Ata2); Peroxiredoxins 5(prd 5); cysteine proteinase inhibitor cystatin c

国家自然科学基金;广东省自然科学基金人发角蛋白;脊髓损伤;大鼠;组织工程学;组织工程化脊髓;桥接物;形态学;行为学;神经元;轴突;神经纤维;髓鞘;星形胶质细胞;少突胶质细胞;小胶质细胞;室管膜细胞;神经干细胞;巨噬细胞;多核巨细胞;溶酶体酶;泛素肽酶系;抑制消减杂交;基因表达;细胞色素氧化酶Ⅲ;氨基酸转运蛋白系统A2;抗过氧化物蛋白5;半胱氨酸蛋白酶抑制剂

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。