抗抑制

Earthworms might be a good bioindicator for the contamination of roxarsone to soil. No significant influence of roxarsone contamination upon the biomass and the reproductive ability of earthworms was found, which indicated that earthworms could tolerate heavy contamination of roxarsone. 2. Effects on the important enzymatic system of earthworms The activities of PROD, BROD and MROD in P450 family were inhibited.

对蚯蚓体内重要酶系统的影响洛克沙胂对P450酶系中的PROD（7-戊氧基异吩噁唑酮-O-脱乙基酶）、BROD（7-苄氧基异吩噁唑酮-O-脱乙基酶）和MROD（7-甲氧基异吩噁唑酮-O-脱乙基酶）的活性都有抑制作用，对MROD的抑制作用最明显；在抗氧化酶系统中，对过氧化氢酶在整个试验进程中都有激活作用，而对其他抗氧化酶有时起激活作用，有时起抑制作用，规律性不明显。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

The mechanism probably is that Pishang can inhibit the growth, movement and adheresion to extracellular matrix and endothelial cell of liver tumor cells, up-regulate the expression of nm23, down-regulate the expression of CD44, MMP-2, and inhibit the tumor neovascular by supressing the vascular endothelial cell, down-regulating the expression of VEGF, damaging the primitive mesenchymal cells and inhibiting neovascular gemmation.

砒霜具有明显的抗肝癌细胞作用，并有一定的抗侵袭与转移作用，其机制可能与其能抑制肝癌细胞增殖、迁移、阻止肝癌细胞与细胞外基质和内皮细胞粘附、上调肿瘤转移抑制基因nm23表达、下调肿瘤侵袭分子CD44、MMP-2表达以及通过抑制内皮细胞增殖、下调VEGF表达、损伤间充质细胞、阻止新生血管芽生等途径从而抑制肿瘤新生血管形成有关。

The results indicated that HDPCs'proliferation was inhibited significantly when they were cultured in DMEM with 50、100μg/mlLPS. Low-dose of Chinese nutgall water extracts( 1.25、2.5、5μg/ml) resisted the inhibitory effect of LPS to HDPCs'proliferation significantly .From the third day , the proliferation activity of HDPCs incubated with 2.5μg/ml Chinese nutgall water extract and LPS increased signicificantly in a time-dependent manner.

结果显示，培养液中加入浓度为50μg/ml、100μg/ml的LPS时，HDPCs的增殖活性受到明显抑制，加入低浓度(1.25μg/ml、2.5μg/ml、5μg/ml)五倍子水提取物能明显拮抗100μg/ml的LPS对HDPCs增殖的抑制作用，2.5μg/ml五倍子水提取物拮抗100μg/ml的LPS对HDPCs增殖抑制作用第3天开始明显增加，并呈时间依赖性。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

But both 20 years and 2 years old of pu-erh tea extracts show a strong inhibitory effects against 2-AAF. In addition, tannic acid, gallic acid, rutin, umbelliferone, and quercetin exhibited stronger antimutagenicities against PhIP than apigenin; while all of these polyphenols displayed inhibitory effect against 2-AAF.

而比较数种多酚类化合物发现，tannic acid、gallic acid、rutin、umbelliferone和quercetin对於抑制PhIP的抗突变作用比apigenin来的有效，但是apigenin和其他多酚类化合物对於抑制2-AAF的抗突变作用则均具有明显的抑制效果。

Cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A.

接著并以活性为导向的分离策略，从樟芝子实体乙醇抽出物中分离出具抗发炎活性成分antrocamphin A，续利用LPS诱导小鼠巨噬细胞（RAW 264.7）产生发炎的模式，解析antrocamphin A之抗发炎机制，结果证实antrocamphin A确可有效抑制一氧化氮自由基和前列腺素的生成，透过蛋白质表现分析得知，antrocamphin A之抗发炎活性是经由抑制一氧化氮生成酵素和第二型环氧酵素的mRNA表现，进而抑制了一氧化氮生成酵素、第二型环氧酵素和核转录因子-κB等酵素之表现。

Through measuring MIC and bacteriostasis diameter, the inhibited abilityof antimicrobials to bacterium and funguses is assessed. Through measuring optical density, inhibited ability of single antimicrobials to algaes si assessed. And, the variation law of antibacterical effect of antimicrobials is researched with variation of time and pH.

通过测定最低抑菌浓度MIC值和抑菌圈直径考察单体抗菌剂对菌类的抑制能力，通过对水样光密度的测定考察单体抗菌剂对藻类的抑制能力，并研究抗菌剂抗菌效果随时间及pH值的变化规律。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。