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Twenty two strains(14 bacteria and 8 actinomyces strains) of antagonistic microorganisms against Fulvia fulva were isolated from vegetable field soil.

从蔬菜地田间土壤中筛选出对番茄叶霉病具拮抗作用的22株菌株,其中14株细菌,8株放线菌。孢子萌发抑制试验表明,细菌X14菌株和放线菌F15菌株的发酵液及其离心后上清液对番茄叶霉病菌孢子萌发抑制率均达100%。

Many microorganisms in fermented dairy products stabilize the bowel microflora, and some appear to have antimicrobial properties.

发酵乳制品中的许多微生物可以稳定肠道内的微生物菌群,而且有抗微生物的性质。

The endophytic bacteria E1R-J is isolated from the root of winter wheat,which has the better effect to take-all,powdery mildew and rust of wheat.

细菌E1R-J是一株对小麦全蚀病、小麦白粉病和小麦条锈病有拮抗作用的小麦内生芽孢杆菌,笔者对其产菌的发酵培养条件进行了初步研究。

The high inoculum levels increased the bacterial growth and shorten the time for antifungal substance production. The powder of rice, soybean cake, yeast, and wheat bran, CaCO3 and KH2PO4 were better nutrients for the bacteria growth and antifungal substance production. There were two stages during the fermentation process at 30℃. The growth of bacteria increased at the first 24 h and the pH of broth rose, but no antifungal activity was observed until 41 h later and reached to the highest level at 114 h.

基本营养物质的初步筛选试验证明:大米粉、豆饼粉、麦麸、酵母粉和CaCO3及KH2PO4是发酵培养基中最佳的营养物质,可满足抗菌物质产生对碳、氮和矿物质的营养需求。B-903的发酵过程可分为两个阶段:在30℃下,24小时内,细菌数量达最大值,pH值由低向高变化,但此阶段检测不出抗菌物质的活性;第二阶段,培养41小时后,抗菌物质活性开始显露,至112小时后达最大值,pH值也逐渐由高变低。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌,在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛,得到6株对青枯劳尔氏菌有较强拮抗作用的细菌,通过摇瓶发酵后利用发酵液,采用改良的琼脂孔扩散实验复筛,获得一株具有强拮抗作用的菌株,命名为Q6号。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

The culture filtrate of strain LN-176 was tested for light absorption at 260nm and 280nm, and the results showed that the protein content in the culture filtrate significantly increased. After ammonium sulphate precipitation, the supernatant and the precipitation were separated to testify their antagonistic activity, and it was found that the precipitation was inhibitory to Ustilago hordeum , but the supernatant not.

LN-176菌株的发酵液在260nm和280nm下进行吸光度测定,结果表明发酵液中蛋白质含量明显升高;发酵液经硫酸铵盐析后,取上清液和沉淀物分别做抗菌试验,发现沉淀物对青稞散黑穗病菌有拮抗活性而上清液无活性。

Their fermentation filtrates had significant inhibitory effects upon the pathogen, with the inhibitory rates as 73.72% and 75.32%, respectively.

sp。 Mali具有较强拮抗作用的菌株,其中菌株B86和B91的发酵滤液在活体上时苹果斑点落叶病的防治效果显著,分别为73.72%和75.32%。

The fermentation conditions of the antagonistic bacteria 12-47 to cotton wilt were studied by using the single-factor method. The main factors affecting fermentation conditions include C and N sources, inorganic salts, pH, temperature, rotation speed.

本实验采用单因素实验的方法在摇床培养的基础上对影响棉花黄萎病拮抗细菌12-47菌株产生拮抗物质的营养因素进行了考察,其中包括发酵培养基的C源、N源、无机盐等。

The results showed that the fermentation liquid from A44 showed strong inhibitory effect on mycelium growth and on spore germination with tower germination rate against pathogenic funguses Alternarina.alternate .

结果表明A44菌株发酵液对烟草赤星病菌、黄瓜枯萎病菌、西瓜枯萎病菌等病原菌有抗菌活性及抑制孢子萌发作用,且这种作用在A44发酵液稀释了160倍后仍然存在;对峙培养试验表明A44菌对植物病原菌有很强的拮杭作用,拮抗机制主要为营养竞争作用、抗生作用等。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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