抗原性

Objective To study the change in the rule of quick innate immune reactivity of fresh blood corpuscles to cells antigen.

目的 研究新鲜血细胞对细胞性抗原快速天然免疫反应的变化规律。

An ELISA(enzyme-linked immunosorbent assay.) was developed for detection of bovine brucellosis by using LPS as antigen purified by Heating - phenol recommended by OIE (office international des epizootics).

采用世界动物卫生组织推荐的热酚法提取的脂多糖作为抗原，建立了牛布氏杆菌病的ELISA方法，对45份血清进行检测，证明本ELISA方法灵敏性较高，经阻断试验和交叉试验，证明特异性较好。

It is located in chromosome 2 (2P13) and has one more intron sequences of BARD1 gene.

结果：55个克隆的核苷酸序列代表45个卵巢癌抗原基因，其中8个是国内外首次发现的在GeneBank中尚未登记的新基因，这些新基因都是具有免疫原性的功能基因。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

We employed yeast one hybrid system developed by Li in 1993 to screen the human liver cDNA library to fish the proteins binding with the HBV large surface antigen promoter DNA.

目前的研究认为表面抗原大蛋白的表达具有肝特异性，在HBV的嗜肝性及复制循环中扮演着重要角色。

It has been hypothesized that destruction of biliary tract in primary biliary cirrhosis would be mediated by autoreactive liver-infiltrating T cells through either cytotoxicity or lymphokine production.

抗原特异性T细胞与原发性胆汁性肝硬化(Primary biliary cirrhosis，PBC)中胆管特异性损伤密切相关，并在PBC的发生、发展中起重要作用。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

A multi-epitope antigen gene pcx was assembled by five highly conserved B-and/or T-cell epitopes, which were selected from C (1-16aa, 132-141aa), E1 (126-135aa), NS3 (139-147aa) and NS5B (768-775aa) protein, as well as a T-cell epitope of tetanus toxin, which was added to enhance T-cell immunity.

针对HCV的高度变异性及免疫特点，在HCV基因产物中优选了5个具有良好免疫原性的高度保守B／T细胞表位，组合成一个多表位抗原基因pcx，这些表位分别来自核心蛋白(1～16aa，132～141aa)，E1(126～135aa)，NS3(139～147aa)及NS5B(768～775aa)，以及破伤风毒素的T细胞表位用以增强T细胞免疫。

C. isoflavone treatments, respectively. All mice were weighed at the beginning and end of the experiment, injected with antigen and induced DTH on time during the period. 21 days later, blood sample, stomach, small intestine, spleen, thymus and sap of abdominal cavity were taken.

于试验期始末称量小鼠体重，试验期内的特定时间注射抗原诱导迟发性变态反应并测耳增厚；试验结束采血，取胸腺、脾脏、胃和小肠并称重，取腹腔液制片。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失