抗原性

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

These results suggest that gastrointestinal Candida colonisation promotes sensitisation against food antigens, at least partly due to mast cell mediated hyperpermeability in the gastrointestinal mucosa of mice.

这些结果提示胃肠诞道念珠菌定植可促进食物抗原的致敏反应，这一作用至少部分是由肥大细胞介导的小鼠胃肠道黏膜通透性增高所致。

Objective To investigate the expression of T cells subgroup and cutaneous lymphocyte-associated antigen in peripheral bloods from patients with psoriasis vulgaris.

目的探讨T细胞亚群和皮肤淋巴细胞相关抗原在寻常性银屑病外周血中的表达情况。

METHODS: The VacA antigen expressed engineering bacterium was induced by lsopropyl-β-D-thiogalactopy-ranoside. The expressed inclusion body was denatured, renatured and dialyzed, and its activity was identified.

利用已经构建的表达VacA抗原的工程菌，用IPTG诱导，表达的包涵体通过系列条件的变性、复性、透析，并经活性鉴定。

This assay has been proved to be specific and sensitive for diagnosis of piglet diarrhoea caused by ETEC and ifentification of the fimbriae type of ETEC.

结果表明，该多重PCR方法具有很好的特异性和敏感性，可用于ETEC性腹泻的辅助诊断及ETEC菌毛抗原分型检测。

Objective The purpose of this study was to investigate the allele frequencies of the human platelet alloantigens 1-16 system (HPA 1-16) in Shanghai unrelated and healthy Han population, and provide the credible basis to look for compatible platelets transfusing patients.

目的 研究上海地区汉族人群人类血小板抗原HPA-1-16系统基因多态性分布，为快速地寻找更合适的血小板输注给与患者提供实验依据。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

Objective To investigate the effect of HLA-specific antibodies, population reactive antibody, on the outcome of renal allografts.

目的 探讨人类白细胞抗原群体反应性抗体对肾脏移植效果的影响。

The optimal conditions for coating antigen concentration and antisera dilution were 0.6μg/mL and 1∶64,respectively.

诱导表达的重组P25蛋白纯化后进行包被，十字交叉滴定试验确定，此ELISA的最适抗原包被浓度为0.6μg/mL，血清最佳稀释度为1∶64，与大肠杆菌、多杀性巴氏杆菌感染鸭血清无交叉反应。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失