抗原性

Objective To obtain the functional fusion protein of rhoptry protein 2(ROP2) and major surface antigen 1 of Toxoplasma gondii in order to further prepare for the diagnosis and polyvalent vaccines of toxoplasmosis.

目的构建编码弓形虫RH株棒状体蛋白2(ROP2)和主要表面抗原1的重组表达质粒，纯化和复性的融合蛋白为弓形虫病快速诊断试剂盒及蛋白质疫苗的研制作准备。

The immunogenicity and protective efficacy of the DNA vaccine is assessed, the vaccine which include cholera toxin B subunit and polyvalent protective epitopes of Plasmodium falciparum, using rhesus monkeys as animal models.

研究了以霍乱毒素B亚基为载体的重组疟疾多价抗原的DNA疫苗在恒河猴中的免疫原性及对相应疟原虫感染的免疫保护作用。

Western blotting analysis revealed that the recombinant protein could react with rabbit anti-African swine fever virus VP73 polyclonal antibody. Fusion expression of African swine fever virus VP73L is helpful to to prepare ASFVserological diagnostic reagent in next work.

所得结果为进一步研究：分离纯化该重组融合蛋白；确证目标抗原蛋白VP73L免疫原性及其特异性，进而达到建立非洲猪瘟病毒的免疫检测方法奠定了必要的材料与技术基础。

Methods Sera derived from 226 SLE cases and 183 healthy controls were tested for ANA and all parameters were compared such as sensi- tivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,nega- tive likelihood ratio,result consistency,rank correlation coefficient and kappa of ANA detected by IFA...

方法同时采用IFA法和ELISA法检测血清ANA共409例，其中SLE 226例和健康对照183名，将两种方法检测结果的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比、阴性似然比、结果符合率、判断一致性κ值及秩相关系数进行比较分析；通过ROC曲线比较两种方法的准确度；结果不一致标本进行可提取性核抗原抗体谱检测分析；同时将IFA法检测不同荧光模型样本分别与ELISA结果进行相关分析。

HBV preS1 antigen is acted as complement of HBeAg and HBV DNA, and a index that judges reactiveness of chronic hepatitis patients.

PreS1抗原可以作为HBeAg和HBV-DNA的补充，也可作为判断慢性乙型肝炎患者病情活动性的一项指标。

To study the expression of proliferating cell nuclear antigen in human gastric neoplasm and its clinical significance. The expression of PCNA was semiquantitatively analysed immunohistochemically in 10 gastric adenomatous polyp and 94 gastric carcinomas.

为了解增殖细胞核抗原在胃肿瘤中的表达及其临床意义，应用免疫组化技术，对胃肿瘤(胃腺瘤性息肉10例，胃癌94例)组织切片中PCNA含量进行半定量检测。

Oral administration with a certain amount of Se could sensitize the response of goat immunocytes to antigen.

山羊灌服一定量的硒，可显著提高其免疫细胞对抗原刺激的反应性。

The serum samples were detected by Indirect ELISA and Indirect hemagglutination test as reference method to assess the value of the recombinant protein in serodiagnosis.

结论1、大量表达并纯化了重组蛋白SjRPS4，并证实其具有良好的免疫反应性，能与日本血吸虫病阳性血清特异性结合，可应用于日本血吸虫感染血清学诊断。2、成功建立了以重组蛋白SjRPS4为包被抗原的间接ELISA法，在日本血吸虫病的免疫诊断中具有良好的应用价值。

Initial adherenced cells mostly are round or short fusiform. Division growth of adherenced cells was shown 72 hours after fully changing suspension(.2)Flow cytometry analysis of the adherenced MSCs indicated good homogenicity of passage cells. More than 95% CD34- and CD44+ demonstrated the cells we get were purified MSCs.

2对贴壁生长的MSCs进行流式细胞仪检测，提示传代的细胞均一性较好，表面抗原CD34表达阴性，CD44表达阳性的细胞均在95%以上，说明得到的是比较纯化的MSCs。

NPC displays a type Ⅱ latent phenotype expresing the subdominant proteins of EBNA1, LMP1 and LMP2 only.

肿瘤细胞中病毒蛋白呈潜伏Ⅱ型表达，即仅表达低免疫原性抗原EBNA1、LMP1、LMP2。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失