抗原性

Only a reaction mice without immunogenicity of substances, called hapten, such as penicillin, sulfa, etc..

只具有反应原性而没有免疫原性的物质，叫做半抗原，如青霉素、磺胺等。

Objective: To evaluate the value of the antigen capture assay in the diagnosis of idiopathic thrombocytopenic purpura.

目的：探讨抗原捕获试验在诊断特发性血小板减少性紫癜中的应用价值。

Objective: Association research of Chinese Han chronic obstructive pulmonary disease and human leucocyte antigen.

目的：探索我国汉族人群人类白细胞抗原等位基因多态性与慢性阻塞性肺疾病的易感性。

Such unresponsiveness to self-antigens and nonpathogenic environmental antigens is called immune tolerance.

所谓「免疫耐受性」系指免疫系统对於某些抗原的不反应性。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

The mycobacterial antigens are not affected by the immune state of the host. In the present study, three different antibodies are used to detect the tubercular antigens in the mononuclear macrophages in CSF and PB.

本实验通过对脑脊液和外周血中单核细胞内三种不同的结核抗原的检测，比较三者对结核性脑膜脑炎诊断的意义，为结核性脑膜脑炎的诊断寻求一种更有效的方法。

There are 11 gene bindings disappear in the normal group, but appear in the model group, and then disappear in the treat group, that indicates the 11 gene bindings are correlated with the epilepsy and Caohuozhimutang. After searching in the Gene Bank, 7 bindings in the 11 gene bindings are homogenic with the 8q31 ribosomal protein gene, 15p12 paraneoplastic neuronal antigen gene, 4q22 diacylglycerol kinase iota gene, xq37 FMR2 protein and 11p11 roudabout homolog 1, and 4 bindings of which are novel gene.

经NCBI美国国家基因库检索，这11条基因中的7条已知基因分别与位于鼠染色体8q31位置的核糖体蛋白、15p12位置的周围肿瘤神经元的抗原(paraneoplastic neuronal antigen)、4p22位置的甘油二酯酶、xq37位置的FMR2蛋白（FMR2 protein）的基因以及以及位于果蝇染色体11p11位置的交叉同族体（roundabout homolog 1）有极高的同源性；有4条基因片段同源性较低，为功能未知的新基因，已经在GeneBank进行了注册，注册号分别CK325391、CK325392、CK325393、CK325394。

Pioneering work by Cohen and coworkers about T cell vaccination in the experimental autoimmune disease, has showed that the establishment of immune response against T cell vaccine, such as anti-idiotype T cells or anti -idiotype antibody, by the way of the upregulation of immune networks, and subsequently functional depression of autoimmune to self-antigen were made by vaccining mice or rate with the attenuated autoreactive T cells .

从研究TCV在防治自身免疫性疾病中的作用机制表明，人为的TCV免疫，建立了拮抗T细胞的免疫应答，使免疫网络产生上调作用，抑制了原有的或即将出现的相关T细胞的功能，由此推测，是否能够把同种反应性T细胞作为TCV，来建立抗同种免疫反应的内环境，诱导抗原特异性的同种免疫耐受或免疫低反应性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。