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Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌,经复壮后选取致病性强的6菌株作为供试菌株,制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验,根据各菌株抗原的交叉反应程度,筛选出抗原交叉程度高的菌株300和343,检测两菌株超抗原的毒性作用,以不同方式制备抗原免疫仔猪,用间接ELISA检测免疫效果,并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验,进行相关分析。

That the four synthetic peptides were able to be efficiently recognized in ELISA by their respective homologous antisera showed they were all antigenic.

结果显示,4个合成多肽均能被相应的抗体识别,具有良好的抗原性;能诱导产生特异性IgG抗体。

Objective:To analyze the genotype of the porin gene in Neisseria gonorrhoea wild strains. Bacterial agglutination has been carried out to verify whether there is an antigenicity change caused by the porin gene mutation.

目的:采用PCR和探针技术分析临床淋病奈瑟菌野生株孔蛋白基因的基因型,并和相应多克隆抗体进行反应以检测基因突变对抗原性的影响。

AIM To investigate the effect of fluid nitrogen cr yopreservation on the MHC antigenicity of aortic homografts.

目的 探讨液氮低温保存对同种异体主动脉移植物抗原性的影响。

Fluid nitrogen cryopreservation has no obvious effect on the MHC antigeniacity of aortic homografts of rats.

液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞MHC类的抗原性没有明显的影响。

Methods:The peptide sequences of human CYP1A2 were analyzed according to its bioinformatics, and synthesized on the basis of hydrophilicity, antigenicity, accessibility and flexsibility. The synthesized peptides were crosslinked with keyhole limpet hemocyanin, which was used to immunize rabbits for production of Ab.

利用生物信息学方法分析CYP1A2蛋白的序列,根据亲水性、抗原性、柔韧性及表面性等指标选择多肽序列,合成CYP1A2多肽,与载体蛋白钥孔戚血蓝素(Keyhole limpet hemocyanin, KLH)偶联,免疫日本大耳白兔制备抗CYP1A2抗体。

Eight polypeptides were isolated from HBsAg and designated as p-1 through p-8 in the order of their decreasing mobilities and have molecular we...

本文报告HBsAg多肽的分离,各个多肽的分子量和它们的抗原性,并对一些主要多肽作为乙型肝炎疫苗的可能性进行讨论,认为HBsAg的多肽有可能作为乙型肝炎疫苗预防乙型肝炎病毒的感染。

moxa fumigation in ward had a certain inactivation effect to HBsAg and HBeAg,but didn't completely inactivate HBV,didn't replace disinfectants on inactivation to virus,and may be used in air disinfection of baby-friendly ward.

为了进一步弄清艾条熏蒸对病毒是否有灭活作用,我们按有关文献方法[3,4],在爱婴区通过艾条熏蒸对乙肝病毒HBsAg和HBeAg抗原性的灭活进行了研究,现将结果报告如下。

Result A strain of Pseudomonas cepacia was detected in 1.6% compound quaternary ammonium disinfectant before application. The amount of the germ was 1.21×105cfu/ml. The strain had the similar resistance to chlorine-containing disinfectants and glutaraldehyde compared with Staphy aureus. The contaminated disinfectant could still thoroughly destroy the antigenicity of HBsAg with 60min's contact after diluted for 5-20 times.

结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近,污染样液除菌后1 000倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果,20倍稀释可完全破坏HBsAg抗原性,和抑制枯草杆菌黑色变种芽胞的生长,1 600倍稀释可抑制白色念珠菌生长,而原液则不能抑制分离菌株的生长。

The nuclear protein encoded by N gene is consist of 422 AA residues (47kDa), Each virion contains 1258 copies that can effectively protect viral RNA from being digested by nucleotidase. The N protein presents a group specificity, it exists in various types and subtypes of VS and plays a very important role in transcript copy. Due to its characteristics of high antigenicity, the N protein can stimulate the body producing nonneutralized antibody.

它可有效的保护病毒RNA免受各种核酸酶的消化。N基因编码的N蛋白呈群特异性,为许多型和亚型所共有,有高的抗原性,刺激机体产生非中和抗体,且在转录复制中担任了重要的角色。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。