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Aim To detect the circulating antigen in sera from experimentally infected animals and patients with trichinosis for early diagnosis and evaluation of treatment efficacy.

目的 检测宿主血清中旋毛虫循环抗原以诊断本病和考核药物疗效。

The sensitivity and specificity of PELA in diagnoses of rat and human trichinosis were studied by Western blot while the antigens of the encysted larvae were used as controla.

应用免疫印迹对比分析PELA和成囊幼虫抗原的组分和它们对人及大鼠旋毛虫病诊断的敏感性和特异性。

Methods The phage displayed antigens of paragonimus were used to examine the sera of patients with paragonimiasis,schistosomiasis,trichinosis and healthy people,and their sensitivity and specificity were acquired;The templates of the mimic antigen were purified to determine the sequence and the sequence homology was analyzed by Blast.

方法建立肺吸虫噬菌体模拟抗原的ELISA,对肺吸虫病人、日本血吸虫病人、旋毛虫病人及健康者血清进行检测,分析其敏感性和特异性;提纯噬菌体模拟肽的DNA模板进行序列测定,以Blast软件分析其序列同源性。

Meanwhile,the indirect agglutination of the serum of 119 trichinosis patients with the trichine-lla chromatography antigen and the sensitive sheep erythrocyte (both were made in this institute)showed that 116 cases presented positive reaction .

同时,应用本院制作的旋毛虫层析抗原致敏绵羊红细胞,采用间接血凝法检测119例旋毛虫病人血清,阳性116例,阳性符合率达97.47%,假阴性3例,占2.53%。

Methods Fungus, trichomonad, germs, anaerobic bacteria Gardinerella was detected for sialidase by chemical reaction; Gold colloid method was adopted to detect the antigen of chlamydia trachomatis.

真菌、滴虫、各种细菌、厌氧菌、支原体采用体外培养和生化反应的方法进行培养和鉴定,加德纳菌采用化学反应的方法检测唾液酸酶,沙眼衣原体采用金标法检测其抗原

Schistosoma japonicum ; triose phosphate isomerase; mimic antigen epitope

日本血吸虫;磷酸丙糖异构酶;模拟抗原表位

Objective To screen the mimic antigen epitopes of the triose phosphate isomerase of Schistosoma japonicum Chinese strain and investigate their immunogenicity. Methods The random phage peptide library PH.D.

目的 筛选日本血吸虫中国大陆株磷酸丙糖异构酶分子的模拟抗原表位,研究其免疫学特性。

Based on the above-mentioned, two kinds of target glycoclusters with different glycoterminus, flexible linkers and different scaffolds were designed, disaccharide glycocluster (including TM1-TM7) and trisaccharide glycocluster (TM8-TM9) respectively, with the purpose of obtaining glycoclusters with high binding affinity to anti-Gal antibody and thus better inhibiting the HAR.

基于上述,针对HAR产生的机制,以人体中天然存在的anti-α-Gal抗体及B细胞表面的相应受体为靶点,以α-Gal抗原末端的二糖Galα1→3Gal和三糖Galα1→3Galβ1-4GluNAc片段为糖基部分,采用柔性的连接臂(氨基保护的2-乙醇胺和3-巯基丙酸)和刚性的芳香骨架及柔性的脂肪链骨架,设计了两类结构新颖的糖簇分子:即氧苷和硫苷键连接的二糖糖簇TM1-TM7)和三糖糖簇(TM8-TM9),目的在于寻找与anti-Gal抗体及B细胞相应受体有较高结合力的糖簇分子,以便有效地抑制HAR反应的发生。

The antigenic reservoir produced a protective rate as high as 80%, which provides a way for developing new vaccine of Trypanosome evansi.

抗原库的最佳免疫保护率达到 80%以上,它的建立为伊氏锥虫疫苗的研制开辟了一条新的途径。

Itproved a trypanosome can underwent antigenic variation.

采用cy鼠克隆和免疫学检测方法分离鉴定了抗原变异体ShTat1.1-ShTat1.9和ShTat2.1-ShTat2.5。

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