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The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

Group A was muscularly injected with normal saline, Grou...

观察C、D组是否感染成功,比较4组家兔的健康和发病情况,观察弓形虫抗原的分布及致病情况。

Objective To evaluate the value of protein chip for clinical detection of multiple Mycobacterium tuberculosis antigen.

目的 验证抗结核分枝杆菌多种抗原的抗体检测蛋白芯片的临床应用价值。

Looking for the specific antigen of mycobacterium tuberculosis is significant for tuberculosis diagnose.

寻找到结核分支杆菌的特异性抗原,对于结核病的诊断具有重要意义。

Methods Human leukocyte antigen-DR expression on skeletal myocyte in 75 cases of neuromyopathy and normal controls was detected by immunohistochemistry.

应用免疫组化SP法系统观察75例神经肌肉疾病患者和外科手术无肌病患者的正常骨骼肌细胞人类白细胞抗原DR位点的表达。

Twelve of the 52 cases with positive immune complex showed negative when tested for the antigens and antibodies separatedly.

在52例循环免疫复合物阳性标本中,12例囊尾蚴抗原和抗体均为阴性。

Objective: To study the expression of EGFR and PCNA protein in human gastric neoplasm and its clinical significance.

目的:研究表皮生长因子受体和增殖细胞核抗原在胃肿瘤中的表达及其临床意义。

Objective To investigate clinical characteristics of optic neuritis and its association with HLA.

目的探讨视神经炎患者的临床特征及与人白细胞抗原的相关性。

The capture of the labeled antibody–analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned.

对产生信号的标记抗体-抗原复合物的捕获,是用抗体固定在已经提到的硝酸纤维素的特定区域内来达到的。

Thus nonage secreted protein is the protective antigen.

因此,早期分泌蛋白是主要的保护性抗原

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