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During contact, a small molecule from the plant, called a hapten, binds to carrier protein in the host.

接触中,被叫做半抗原的,来自植物的一种小分子,与宿主的载体蛋白结合。

Only a reaction mice without immunogenicity of substances, called hapten, such as penicillin, sulfa, etc..

只具有反应原性而没有免疫原性的物质,叫做半抗原,如青霉素、磺胺等。

The UV scanning and infrared spectrum results showed that the hapten and the carrier protein were successfully coupled to BSA and OVA, with the combined ratios of 3.8∶1 and 5∶1, respectively.

紫外扫描与红外图谱分析结果表明,半抗原与载体蛋白偶联成功,与BSA、OVA的结合比分别为3.8:1和5.0:1。

The development of enzyme immunoassays for the determination of pyrethroid residues were reviewed,including hapten design ,antibody preparation,and immunoassay establishment and applications.

并对拟除虫菊酯类农药通用免疫半抗原设计及多残留酶免疫分析技术的发展趋势进行了探讨。

The horseradish peroxidase tagged antibody was prepared by means of modified NaIO4 method and the HRP tagged hapten was prepared by means of mixed anhydride method.

以辣根过氧化物酶采用改良的过碘酸钠法标记抗体、混合酸酐法标记半抗原

Catalytic antibodies ; abzymes ; hapten design ; random mutagenesis ; selection

催化抗体;抗体酶;半抗原设计;随机突变;筛选

Methods:YCP was coupled to bovine serum albumin to construct three neoglycoprotein s which are different in their degrees of substitution(DSs;≈3,≈6,or ≈10 mol of BSA carrier/mol of YCP hapten,respectively),and their immunogenicities were evaluated in mice following subcutaneous immunization,respectively.

将YCP与牛血清白蛋白共价偶联,获得取代度分别约为3、6和10的3种拟糖蛋白,经皮下分别接种ICR小鼠,测定免疫血清中多糖YCP的特异性抗体IgM和总IgG水平,以及半抗原和载体蛋白的特异性IgG亚型。

It has good specificity and sensitivity by a competitive inhibition enzyme-linked immunosorbent assay. The hapten was linked with enzyme and determination method was established primarily that based on competitive ELISA. The limit of this method was 1ng/ml. The study will be useful for studying the immunoassay in the determination of CL residues.

抗体的亚类为IgM,腹水及细胞上清间接ELISA效价分别为1:20 480和1:320,通过竞争阻断实验证明其具有良好的特异性及敏感性,将半抗原经酶标后应用竞争ELISA方法初步建立了CL的检测方法,检测限可达1ng/mL,为进一步制备CL残留检测试剂盒打下了基础。

Both kinds of immunogen can successfully elicit 36AA specific antibody response in rabbits, which have different immune characteristics. To couple the hapten with vector proteins is a quicker and more effective way to prepare the immunogen.

偶联蛋白方法与基因工程方法制备的免疫原均可成功刺激家兔免疫系统产生针对36肽半抗原的有效抗体,但两者具有较为不同的免疫特性,偶联蛋白方法更加迅速有效。

Using hapten penicillin G as a model, the nanogold in size of 9 nm was used to label rabbit anti-penicillin G antisera to obtain an immunonanogold probe for PG.

以半抗原青霉素G为模型,用粒径为9 nm的金纳米微粒标记羊抗兔青霉素噻唑蛋白抗体制备了青霉素G的免疫纳米金共振散射光谱探针。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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