抗原

In the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic S helix of AOX. were synthesized by solid-phase method. The polypeptide was coupled to a -chymotrypsinogen, and the antibodies were obtained through immunizing rabbit by injecting this complex.

第一部分，我们按照交替氧化酶(Alternative Oxidase，AOX)位于线粒体内膜外侧亲水区S螺旋的氨基酸序列，用固相法合成由12个氨基酸残基组成的多肽，将此多肽与α-牛胰凝乳蛋白酶原A相连，用作半抗原，免疫家兔制备人工合成12肽的抗体。

Recent work has shown that the free-liing and easily cultiated ciliate Tetrahymena can be transformed and express the i-antigen.

近来研究表明，自由生活且易于培养的四膜虫被转化后能表达i抗原。

Experimental methods: pathological slices: To observe the changes of pathomorphology and measure the circular area of internal elastic lamina , area of neonate intima and vascular lumen, the intimal proliferatory index and percentage, the index of lumen stenosis and positive index of proliferating cell nuclear antigen through the specimens stained by HE and immunohistochemistry.

实验方法：病理切片：进行HE染色和免疫组织化学染色，分别观察病理形态学改变；测定和计算受损血管内弹力膜环绕总面积、新生内膜面积、管腔面积、内膜增生指数、内膜增生百分比、管腔狭窄指数和增殖细胞核抗原阳性指数；观察MMP-2、MMP-3、TIMP-1和PDGF-BB的免疫组化表达。

Antibodies circulate in the blood and their job is to get rid of antigens.

抗体流通，在用鲜血和他们的工作是要摆脱抗原。

The inverse method of indirect coagulability test is commonly used to examine the hepatitis B surface antigen in medical check-up.

用反向间接血凝试验测定乙型肝炎表面抗原是体检普查工作中的一项基本内容。

The coagulase in filtrate of Staphylococcus aureus was a complex of sugar and protein, with antigenic activity, and might play a role in the function of filtrate of Staphylococcus aureus.

金黄色葡萄球菌滤液中凝固酶为一类糖与蛋白复合物，具有抗原活性，可能在金黄色葡萄球菌滤液的功效中发挥作用。

During liquefaction of the ejaculate, the semen coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2) are degraded to low molecular mass fragments by kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen.

射精后精液液化的过程中，精液凝结蛋白semenogelin I（SEMG1）和semenogelin II（SEMG2）被血管舒缓素相关肽酶3（kallikrein-related peptidase 3，KLK3）降解成小分子量片断。KLK3也被称作前列腺特异抗原。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

It results from an inappropriate immune response, in genetically susceptible individuals, to microbial antigens of commensal microorganisms.

他起因不适当的免疫应答、遗传易感个体及共生的微生物抗原，并可被特定的环境因素所诱发。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。