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Objective This study is to analyze the antigenicity of the NS3 proteins of Chinese HGV and their potential use in the serological diagnosis.

目的 研究HGV NS3区基因产物的抗原性,并探讨NS3蛋白在血清学检测中的应用。

Native scaffold materials carry good biocompatibility and low antigenicity, but their sources are little.

天然支架材料有良好的生物相容性、低抗原性,但来源相对少。

Since the embryonic cells possess lower antigenicity and stronger proliferation capability, it is hoped that they can be used as a novel carrier substitute in tissue engineering research.

由于胚胎细胞具有较低的抗原性和较强的增殖能力,期望其能为组织工程研究与应用开辟新的载体材料。

Western blotting showed that the recombinant protein had good antigenicity.

Western印迹分析结果说明该基因在大肠杆菌中的融合表达产物rSj423具有良好的抗原性。

Western blot was used to identify the antigenicity and immunoreactivity of rFimA.

采用Western blot鉴定rFimA抗原性和免疫反应性。

The key of immunological contraception is to discover a sperm antigen, which is tissue-specific and has the intense antigenicity.

应用疫苗进行避孕的一个最关键的问题是能发现一个精子特异的,且具有强免疫原性的抗原

Its antigenicity was identified by Western blot.

Western blot鉴定重组HpaA蛋白的抗原性。

Abstract] AIM: To construct phage antibody library with predetermined CDR3 and to screen humanized Fab of antihuman integrin ανβ3 monoclonal antibody by epitope guided selection.

摘 要]目的:构建预设CDR3基因的噬菌体抗体库,通过抗原表位导向选择方法筛选抗人整合素ανβ3单克隆抗体人源化Fab。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01);若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%,特异性为94.3%;4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05),且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P<0.01);若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性,则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%,特异性为94.3%;4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P<0.05,且大于各单个抗体检测敏感性。

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