抗原

Much more prolongation ofsurvival term and improvement of survival rate,as well as appearance of a great dealof lymphocytes around pulmonary metastatic nodes and less pulmonary metastaseswere also observed in murines treated with activated、tumor antigen-pulsedmacrophages contrast to those treated with IL-2 gene-transfected macrophageswithout prior pulse of tumor antigen in vitro.

肿瘤抗原冲击的IL-2基因转染巨噬细胞治疗组小鼠期存活明显延长，肺转移结节明显减少，局部病理显示，肺肿瘤周围出现大量淋巴细胞浸润，我们推测这可能是因为抗原冲击的活化巨噬细胞除了增强对肿瘤细胞直接的杀伤效应外，还对其进行处理加工，将免疫信息传递给周围的淋巴细胞，从而引起其增殖活化，并浸润肿瘤组织中。

Objective To study the effects of 20/21 bp partial deletion mutation (from nt 1 748 or nt 1 747 to nt 1 767) in the core promoter region of hepatitis B virus genome complicated by precore stop condon mutation at nt 1 896 on the expression of the viral antigens.

目的为研究乙型肝炎病毒核心启动子20/21bp部分缺失(nt1748/1747至nt1767)及同时存在的A1896点变异对病毒抗原表达的影响。方法利用前期构建的HBV全基因的重组载体转染HepG2细胞后，对病毒抗原进行ELISA检测及Western-blotting分析。

It is suggested that six OMPs ofV.parahaemolyticus ompV, ompW, ompK, ompU, psuA and pvuA, are immunogenicduring in vivo infection, and may be of great significance for vaccine development.Especially, fish group immunized with recombinant ompK achieved a high protection of90%. For the wide expression of this conserved protein among vibrio species, ompKmust be paid enough attention for vaccine development against vibriosis.

研究结果显示了6种副溶血弧菌外膜蛋白ompW、ompV、ompK、ompU、psuA和pvuA具有良好的免疫原性，是宿主免疫应答的保护性抗原；尤其是ompK单个蛋白免疫组获得90％的保护率，由于该蛋白是弧菌中广泛存在的保守性蛋白，极有可能作为制备亚单位疫苗等高效弧菌疫苗的候选抗原。

CD58, also was called lymphocyte function-associated antigen 3, is a surface glycoprotein expressed on many cell types, binds to the T lymphocyte surface glycoprotein CD2. This interaction is important in mediating T lymphocytes with target cells and antigen-presenting cells, and the T lymphocyte rosetting with erythrocytes. It plays an important role in mammal immune system.

CD58又称淋巴细胞功能相关抗原-3(Lymphocyte Function associated Antigen-3， LFA-3)，是存在于机体内多种细胞及血小板表面的一种糖蛋白，它可与CD2结合，从而介导T细胞与抗原递呈细胞等的黏附，此外，还可介导T细胞与绵羊红细胞形成Et花环，在机体免疫系统中具有重要作用。

This fragment, which consists of the chicken-alpha globin gene (5′-MAR)、mouse-metallothionein-Ⅰ and human insulin-like growth factor-Ⅰ, was microinjected into male pronuclei of 468 rabbit 1-cell embryos, and injected embryos were transferred into the oviduct of 36 recipients.

为了检测转基因的表达，我们用〓标记的hIGF-1作标记抗原，hIGF-1作标准抗原与hIGF-1抗血清（美国的Susan和Underwood提供）进行竞争性免疫结合反应〓首次建立了人胰岛素样生长因子（IGF-1）的放射免疫测定方法。

Immunity should the answer is the reaction that the machine body resists the original incitement, also is resist the original material to carry on identifying and expeling of a kind of biology process.

免疫应答是机体对抗原刺激的反应，也是对抗原物质进行识别和排除的一种生物学过程。

At the third passage, BMSCs showed the typical polar swirl morphology. BMSCs were negative for CD34 and CD45, but positive for CD29 and CD44. Following induction, alkaline phosphatase staining, Oil red staining and toluidine blue staining produced a strong reaction in cells.

第3代骨髓间充质干细胞形态单一均匀，呈典型的极性漩涡状生长，不表达造血前体细胞标志抗原CD34和白细胞标志抗原CD45，表达整合素家族成员CD29和黏附分子CD44，经诱导后碱性磷酸酶染色、油红O染色和甲苯胺蓝染色均呈阳性。

The optimum condition of the ELISA are set as follows: 1ug/mL of NFLX -HSA(norfloxacin -human serum albumin) conjugation used as a coated antigen dissolved in carbonate buffer solution(CBS,pH 9.6,0.05mol/L), 0.5 % glutin in PBS(pH7.4,0.01mol/L) as a blocking agent post-coating, horse radish heroxidase-labeled sheep anti-mouse IgG(HRP-anti IgG) diluted by PBS(pH 7.4,0.1mol/L) in 1:1000 as a secondary antibody, and TMB (3,3",5,5"-tetramethyl benzidine dihydrochloride) as a sbustrate.

成功建立了检测抗诺氟沙星抗体的间接ELISA方法，其主要条件如下：pH9.6、0.005mol／L的碳酸盐缓冲溶液为包被液，1μg／mL的连接抗原NFLX-BSA(诺氟沙星-牛血清白蛋白)为包被抗原，1％明胶为封闭液，1：1000稀释的HRP酶标记的羊抗小鼠抗体作为二抗，TMB(3，3，5，5-四甲基联苯胺)作为底物。

The CP15,CP15/60 and CP23 on the sporozoites of Cryptoporidium parvum are major surface protein which are studied in the new pattern vaccine and developed a serologic detection method for cryptosporidiosis.In the experiment,prokaryotic and eukaryotic expression vectors of three genes were constructed by recombinant DMA technique and expressed in E.coli and Hela cells respectively.

微小隐拘于虫（pSporndum pMum，Cp）子抱于表面蛋白 CP15、CP15／60和CP23是微小隐抱子虫主要的表面抗原，它们既是人们研究隐抱于虫新型疫苗的主要对象，也是建立隐抱子虫血清学检测方法的首选抗原。

MS1-5 directed against CSP could confer protection to mice or rat against a non-lethal inoculation of parasites by inhibiting the invasion of sporozoites into hepatocytes and neutralizing their infectivity.

用MS1-Sepharose4B亲和层析柱自SP可溶性蛋白中提取分子量为40Kd的环孢蛋白，具有与全虫抗原相同的免疫原性，而且显示其抗原决定簇表位的重复性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。