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Then, the hapten LBc and LBs was conjugated to bovine serum albumin with CDI method to form immuno-antigen; the haptens LBc, LBs and LBy was conjugated to ovalbumin with mixed anhydride method to form the the coating antigens.The new zealand rabbits were immunized by conjugate of LBc-BSA and LBs-BSA, and titres of anti-bifenthrin serum (5.12×10~4, 3.2×10~5) were determined by non-competitive indirect enzyme-linked assay procedure.

通过碳二亚胺法将LBc和LBs交联于牛血清蛋白作为免疫抗原,通过活泼酯法将分别LBc、LBs和LBy交联于卵清蛋白作为包被抗原,LBc-BSA和LBs为免疫原制备了联苯菊酯的兔抗血清,非竞争间接酶联免疫吸附分析方法测得其效价达5.12×10~4和3.2×10~5。

Two haptens of mono-p-nitrophenyl phosphonates were designed and synthesized according to the structural features of the substrate and its hydrolytic transition state. The phosphonate haptens were coupled to the carrier proteins bovine serum albumin and keyhole limpet hemocyanin .

以对硝基乙酸苯酯水解反应中间体的结构信息进行分子设计,经过Arbzov等五步化学反应合成了二种对硝基苯基类膦酸酯半抗原,将其与载体蛋白(牛血清白蛋白BSA和钥孔〓血兰素KLH)偶联,制得了具不同抗原价(10~60)的半人工合成全抗原

Hepatites A, B and CHepatitis B surface antigen has been detected in the milk of HBsAg-seropositive women, and possibly, small amounts of blood might be ingested by the newborninfant during breastfeeding, from nipple injuries, even if these injuries are small.

血清表面抗原阳性的母亲在乳汁中能检查到乙肝的表面抗原,在婴儿进行母乳喂养的时候存在由于母亲奶头皲裂甚至是小伤口而导致婴儿进食了少量的母亲的血液的可能。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was(88.7±4.3)%,(84.8±2.0)%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was(89.4±3.5)%,(81.9±2.7)%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

RESULTS Expression rate of MHCI antigen of cult ured smooth muscle cells and fibroblast was (88.7±4.3)%,(84.8±2.0)%respectively; and th at of the revived smooth muscle cells and fibroblasts of aortic homografts after cryoprese rvation was (89.4±3.5)%,(81.9±2.7)%respectively.

结果正 常培养的大鼠主动脉平滑肌细和成纤维细胞MHC Ⅰ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHC Ⅰ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

With the establishment of animal models of aortic homograft trans-plantation,using immunohistochemical staining,the differ-ence of the expression of MHCⅠantigen and MHCⅡanti-gen was detected between fresh aortic homografts and cryop-reserved aortic homografts.

建立大鼠同种异体主动脉移植模型,采用免疫组织化学实验研究新鲜主动脉和低温保存的主动脉同种异体移植后MHCⅠ类抗原和MHCⅡ类抗原表达的区别。

"Yes." First Affiliated Hospital of Henan College of Traditional Chinese Medicine Obstetrics and Gynecology of a chief physician, said Wang Lina, if both husband and wife blood group incompatibility, the fetus may be obtained from the genetic father of the missing mother of blood group antigen, blood group antigens of red blood cells that once the mother, mother will produce antibodies, which antibodies can enter the fetus via the placenta, the baby will cause the destruction of red blood cell hemolysis, manifested as jaundice, anemia and other serious symptoms may lead to cerebral palsy; If severe intrauterine hemolytic disease will result in miscarriage or stillbirth.

&是的。&河南中医学院一附院妇产科主任医师王丽娜说,如果夫妻双方血型不合,胎儿就可能从父亲身上遗传获得母亲所缺少的血型抗原,这种血型抗原的红细胞一旦进入母体,母体会产生抗体,而这种抗体可经胎盘进入胎儿体内,就会引起宝宝的红细胞被破坏而溶血,表现为黄疸、贫血等,症状严重可能导致脑瘫;如果在宫内出现严重的溶血症,还会导致流产或死胎。

METHODS: The biological meniscus was prepared with antigen-free technique, while some porcine meniscuses without management were also adopted.

自制去抗原生物型半月板,另取未经过去抗原处理的同批猪的半月板。

The parthenogenetic blastocysts and morulas were seeded on MEF feeder respectively to examine the primary ICM appearance and compare the derivation rate, Results: Twelve batches of mouse parthenogenetic embryonic stem cells were established.

结果:共建立了12株小鼠孤雌胚胎干细胞系,这些细胞SSEA-1抗原阳性,SSEA-4,TRA-1-81,TRA-1-60表面抗原阴性,具有AKP活性,保持正常染色体核型,体内外分化分别形成畸胎瘤和拟胚体。

Although macrophages had been pulsedwith tumor antigen in group of cell contrast and LacZ gene contrast group,adoptiveimmunotherapy had no influence on treated murines,because macrophages could notexert their ctotoxicity and antigen presentation function until they must be activatedfirst.

此外,巨噬细胞必须活化后才能发挥其效应和抗原提呈,因为未处理组和LacZ基因转染组虽经肿瘤抗原冲击,检测指标与质量效果均无变化。

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