抗原

1 We succed in constructing the eukaryon expression vector: pcIiSa, which is consisted of the CD74 and HBsAg"a"fuse gene; 2 The hepG2 cell transfected pcIiSa can expression the HBsAg.

1本研究成功构建乙肝表面抗原a位点基因与CD74分子基因融合的真核表达载体：pcIiSa；2该载体可以在肝癌hepG2细胞系中表达出乙肝表面抗原。

As the executors of antigenpresentation,the function of presentation cells are specially more important.

抗原递呈过程的重要功能执行者—抗原提呈细胞的功能也就显得尤为重要。

Highly active, small-molecule furin inhibitors are attractive drug candidates to fend off bacterial exotoxins and viral infection. Based on the 22-residue, active Lys fragment of the mung bean trypsin inhibitor, a series of furin inhibitors were designed and synthesized, and their inhibitory activity toward furin and kexin was evaluated using enzyme kinetic analysis. The most potent inhibitor, containing 16 amino acid residues with a Ki value of 2.45×10~(-9) M for furin and of 5.60×10~(-7) M for kexin, was designed by three incremental approaches.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

PC84045 human lung carcinoma cells (PC84045 HLCCs) were mixed with the BM cells activated by anti-CD3 McAb and IL-2 and then CD3-ALTO4 bi-specific McAbs was put into the cell suspension. The mixed cells were cultured for 3 days in presence of IL-2 and IL-3. Tumor cell colony formation method in half-solid culture system was used to assess the purging efficiency of the activated T cells to PC84045 HLCCs.

先用抗CD3单抗和IL-2激活肺癌患者骨髓中的T淋巴细胞，然后按不同比例将人肺癌细胞株PC84045细胞掺入到骨髓细胞中，再加入能同时识别CD3抗原和人肺癌细胞表面抗原的双特异抗体CD3-ALTO4，在IL-2和IL-3存在下共同培养3d后测试。

A bacterial protein conjugated to small antigenic determinants such as peptides or other small haptens.

本发明涉及一种与小的抗原决定簇结合的细菌蛋白，如肽或其它小的半抗原。

Pesticides ; haptens ; artificial antigens ; design ; synthesis ; progress

农药；半抗原；人工抗原；设计；合成；进展

Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on.

由于半抗原并没有占据所有可利用的表位，因而我们设想，特异的半抗原抗体在天然形成或在实验室合成时，肯定具有一些曾经被用来对其进行筛选的载体蛋白的某些特征。

On the basis of the initial mechanism of deiodinase and the idea of hapten design derived from the method of"hydrophobic pocket modification", three haptens of thyroxine derivatives, o-methyl-〓, o-benzyl-〓 and o-p-nitro-benzyl-〓 with successively-raising hydrophobicity, were designed and synthesized, and their structures characterized.

以天然脱碘酶的初步催化机制和疏水腔修饰法的半抗原设计思想为依据，设计合成了三种疏水性不同的甲状腺素衍生物半抗原：o-methyl-〓，o-benzyl-〓和o-p-nitro-benzyl-〓，并对其结构进行了表征。

Class-specific polyclonal antibodies for O,O-diethyl phosphorothioate pesticides had been generated. A series of generic haptens were synthesized and coupled to bovine serum albumin and ovalbumin for immunogens and coating antigens, respectively. New Zealand rabbits were immunized with immunogens to obtain corresponding polyclonal antibodies.

通过分析硫代磷酸二乙酯类农药的结构特点，设计并合成了系列半抗原；采用活泼酯法将半抗原分别与牛血清蛋白和卵清蛋白偶联制备了系列免疫原和包被原；通过免疫新西兰大白兔获得了相应抗硫代磷酸二乙酯类农药的类特异性抗体。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。