抗原

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Objective To obtain the recombinant protein of an antigen gene Ts88 of Trichinella spiralis and identify the characteristics of the recombinant protein.

目的 原核表达旋毛虫抗原基因Ts88，并对重组蛋白的抗原性进行鉴定。

Objective To express the antigen gene Ts21 of Trichinella spiralis , purify the recombinant protein and test its antigenicity.

摘要目的原核表达旋毛虫相对分子质量21 000抗原基因（Ts21）并对重组蛋白进行纯化和抗原性鉴定。

Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.

获得具有抗原性的旋毛虫抗原编码cDNA分子

This study is design and preparation of active peptide analogues of mung bean trypsin inhibitor Lys fragment, Trichosanthes trypsin inhibitor I, carcinoembryonic antigen peptide and aspartame using solid and liquid phase peptide synthesis methods.

本论文研究活性多肽类似物的设计、制备和功能，分为四个部分，采用多肽固相和液相合成的方法，分别合成了活性多肽绿豆胰蛋白酶抑制剂 Lys片段长链16肽、天花粉胰蛋白酶抑制剂、癌胚抗原肽、甜味肽等一系列类似物，并测定了这些类似物的生物活性，有以下新的研究结果：1、furin酶抑制剂的设计、制备和功能；2、芳香氨基酸芳环之间的π-π共轭能部分补偿二硫键的作用；3、癌胚抗原肽Gly4被L-Pro替代可增加与HLA-A2分子的结合；4、肽键对甜味肽保持甜味是必须的。

His team's research, as well as other laboratories have confirmed that microbial or other material from the antigen peptide, the surface of antigen-presenting cells HLA molecules and T cell receptor recognition of mutual contacts and the formation of a trimolecular complex-mediated of the occurrence and development of rheumatoid arthritis.

他所在的课题组以及其他实验室的研究均证实，来源于微生物或其他物质的抗原多肽，抗原呈递细胞表面的HLA分子以及T细胞受体相互接触和识别形成一种三分子复合物介导了类风湿性关节炎的发生和发展。

Considering undividing cells might be transfected with adenovirus vector andmacrophages could be activated by IL-2,we transfected mIL-2 gene into the freshlyisolated peritoneal macrophages with recombinant adenovirus,and pulsed with tumorantigen in vitro,in order to enhance the immune effector function as well as theantigen presentation function simutaneously,and acquired more effective actions ofantitumor.

本研究选用可转染非分裂细胞的缺陷型腺病毒为载体，将活化巨噬细胞的小鼠IL-2基因转染至小鼠腹腔巨噬细胞，并在体外行肿瘤抗原冲击，以期在增强其免疫效应功能的同时充分发挥其抗原提呈功能，从而取得更好的抗瘤效果。

Meanwhile, positive human sera of HCV challenged vaccina ted monkeys after 6 weeks immunized, ALT value rose transiently in monkeys vacci nated by PCX. It was positive in monitoring RNA of HCV using RT-PCR in monkey s era. The result demonstrated that Rhesus monkey immunized by multi-epitope PCX could elicit high-level immune responses.

近年来，由于多表位抗原概念的兴起，在针对EB，HIV等病毒感染的疫苗研究中引入了多表位抗原疫苗［门，这种思路使对HCV疫苗的研究有了新的切入点，F.r.i［'］等用 HCV.H77株 E蛋白的多个表位所制备的兔血清与HCV

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

Objective: To find out the major antigenic molecules of radiation-attenuated cercariae, and provide some useful candidate antigens for developing schistosomiasis vaccine.

目的：寻找照射致弱尾蚴中起免疫保护作用的抗原分子，为血吸虫病疫苗研究提供新的候选抗原。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。