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Leukemia ; myelocytic ; acute ; interleukin 1; lymphocyte function associated antigen 1; macrophage 1 antigen

白血病;粒细胞;急性;白细胞介素1;淋巴细胞功能相关抗原1;巨噬细胞1抗原

3Rhe distribution and change of proliferating cell nuclear antigen in seminiferous tubule: The positive staining of proliferating cell nuclear antigen was found in slices of all groups, the immunological positive products were located at karyon, usually in brown yellow granules, only a small part of them in diffuse state; the immunological positive cells were mainly the spermospores, besides a small quantity were spermiocyte, supporting the cell and myoid cell nuclear to be stained.

3增殖细胞核抗原在生精小管中的分布及变化:各组切片均可见增殖细胞核抗原阳性染色,免疫阳性产物位于细胞核,通常呈棕黄色颗粒,小部分呈弥散状;免疫阳性细胞主要是精原细胞,另外还有少量初级精母细胞、支持细胞和肌样细胞核着色。

Methods 84 nasopyaryngeal carcinoma patients,57 nasitis patients and 60 healthy control were selected. EBVCA-IgA and EBNA-IgA in serum were determined to evaluated the diagnostic value of the combined determination of the two indexes.

分别采用免疫酶法和ELISA间接法检测60例正常人,57例鼻炎患者和84例鼻咽癌患者血清中EB病毒壳抗原IgA和EB病毒核抗原IgA,评价两项指标联合检测对鼻咽癌的诊断价值。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原,注射新西兰白兔制备出效价高达1:32000的多克隆抗体作为第一抗体,第二抗体为辣根过氧化物酶标记的羊抗兔IgG,通过交叉稀释法确定抗原、一抗及二抗的最优反应条件,建立酶联免疫吸附分析方法体系,病毒检测灵敏度达30ng。

Prostatic neoplasma ; Carcinoma ; Immunohistochemistry ; PSA ; PCNA

前列腺肿瘤;癌;免疫组织化学;前列腺特异抗原;增殖细胞核抗原

METHODS: mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC,非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞,用自体乳腺癌细胞冻融抗原刺激DC,并和TDLNC共培养,以诱导肿瘤抗原特异性CTL。

METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGMCSF and rhIL4 to induce DC. The nonadherent cells were cultured with IL2 to induce tumor draining lymph node cells.

以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC,非贴壁细胞与IL2共培养后诱导成为肿瘤区域引流淋巴结细胞,用自体乳腺癌细胞冻融抗原刺激DC,并和TDLNC共培养,以诱导肿瘤抗原特异性CTL。

Objective To express and characterize the C terminal fragment of HEV ORF2. Methods The gene encoding C terminal 128 amino acid fragment of HEV ORF2 was inserted into nonfusion expression vector pBV220. The resultant pBVORF2.3 was transformed into E.coli strain DH5α.

用pBV220载体进行抗原的非融合表达,包涵体经变性凝胶过滤层析及离子交换层析纯化后透析复性,以Western blot、ELISA、动物免疫与抗原捕获RT-nPCR等方法研究其免疫学特性。

To study the relationship between the expression of HLA molecule and clinicopathological significance in oesophageal carcinoma.

人类白细胞抗原是免疫系统中的重要分子。它参与机体递呈抗原,决定免疫细胞对肿瘤的识别和杀伤,它与肿瘤的关系一直受到关注。

Sera of human infected with Clnorchis sinensis (509 cases), Opisthorchis viverrini (47 cases), Paragonimus westermani (20 cases) Schistosoma japonicum (14 cases) and serum of 163 healthy students were used for ELISA.

方法制备华支睾吸虫的粗抗原和分泌-排泄抗原,以ELISA方法对华支睾吸虫感染患者509人、麝猫后睾吸虫感染者47人、卫氏并殖吸虫感染者20人、日本血吸虫感染者14人以及健康人血清163人,检测血清特异性IgG。

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