抗原

MethodAmoxicillin were coupled with bovine serumalbuminand oval buminby using N-hydroxysuccini mide actived ester method to prepare the i mmune antigen AMX-BSA and detection antigen AMX-OVA.The adult rabbits were immunized with AMX-BSAto obtainthe polyclonal antibody with high titer.

方法采用N-羟基琥珀酰亚胺活性酯法将阿莫西林分别与牛血清白蛋白、卵清蛋白偶联，制备免疫抗原AMX-BSA和检测抗原AMX-OVA，用AMX-BSA免疫成年兔以获得高效价的多克隆抗体。

Many methods on extraction and separation are determinated by different characteristics of soybean major antigen protein,such as isoelectric precipitatio,cryoprecipitate,salt fractionation,ionic strength and immunization and so on.Influential factors of extraction and isolation include pH of buffer solution,ionic strength,moity and so on.

大豆主要抗原蛋白不同的特性决定其提取和分离方法很多，主要有：等电点沉淀法、冷沉淀法、盐析法、按离子强度不同而沉淀分离法、免疫法等，提取和分离过程中影响大豆主要抗原蛋白分离的因素包括提取缓冲液的pH、离子强度、组成成分等。

It can induce complete immune responses, provide heterologous cross protection, and can be easily prepared as multivalency vaccine. On the other hand, compared with traditional vaccines, live attenuated Salmonella typhimurium S.

而与传统疫苗相比，活减毒鼠伤寒沙门菌这一新型口服疫苗载体释放系统亦具有无需纯化抗原，无需佐剂，可避免抗原在胃内的降解和变性等优点。

Results The re158rodents belonged to3genus in three townships of Lanping County were captured and Rattus norvegicus caught in residential areas accounted for90.09%.Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.Hattanvirus was not detected from other animals.The IgG antibody positive rate to Hattanvirus in the serum of hosts of rodents and human population were12.20%and7.00%.

结果 兰坪县3个乡镇共捕获5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势种；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕获的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕获的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

Results There158rodents belonged to3genus in three townships of Lanping County were captured and Rattus norvegicus caught in residential areas accounted for90.09%.Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.Hattanvirus was not detected from other animals.The IgG antibody positive rate to Hattanvirus in the serum of hosts of rodents and human population were12.20%and7.00%.

结果 兰坪县3个乡镇共捕获5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势种；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕获的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕获的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

Results There158rodents belonged to3genus in three townships of Lanping County were captured and Rattus norvegicus caught in residential areas accounted for90.09%.Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.

结果 兰坪县3个乡镇共捕获5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势种；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕获的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕获的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

Results There158rodents belonged to3genus in three townships of Lanping County were captured and Rattus norvegicus caught in residential areas accounted for90.09%.Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.Hattanvirus was not detected from other animals.The IgG antibody positive rate to Hattanvirus in the serum of hosts of rodents and human population were12.20%and7.00%.

结果 兰坪县3 乡镇共捕获5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕获的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕获的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.Hattanvirus was not detected from other animals.The IgG antibody positive rate to Hattanvirus in the serum of hosts of rodents and human population were12.20%and7.00%.

结果 兰坪县3个乡镇共捕捉5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势种；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕捉的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕捉的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

Apodemus agrariuswas predominant in the field accounted for63.16%.The infectious rates of Hattanvirus in Rattus norvegicus and Suncus murinus were7.69%and25.00%,respectively.Hattanvirus was not detected from other animals.The IgG antibody positive rate to Hattanvirus in the serum of hosts of rodents and human population were12.20%and7.00%.

结果 兰坪县3个乡镇共捕获5属158只啮齿动物，其中居民区中褐家鼠的构成比为91.09%，为优势种；野外高山姬鼠的构成比为63.16%，为优势种。3个乡镇中仅见中排乡捕获的褐家鼠汉坦病毒抗原的感染率为7.69%（5/65），臭鼠句鼠青为25.00%（1/4），其他捕获的动物中未查出汉坦病毒抗原；宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%（5/41），其中褐家鼠的阳性率为12.50%（5/40）；人群中汉坦病毒IgG抗体阳性率为7.00%（3/43）。

AbstractsZCH-2B8a (IgG2a ) is a novel monoclonal antibody generated in laboratory of Children Hospital of Medical College, Zhejiang University recently using human myeloblastic leukemia cell line KG1a as immunogen.

ZCH-2B8a (IgG2a )是作者研究室最近采用髓细胞性白血病细胞系KG1a作为免疫原自行研制的鼠抗人造血细胞分化抗原的单克隆抗体，已提交第8届国际人类白细胞分化抗原协作组会议（HLDA8）鉴定。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。