抗原

In double labell the high contrast substrates of enzym should be used.

因而作者认为，在进行胰岛内分泌细胞的双重免疫组化染色时，一染后可经PBS漂洗后直接进行第二种抗原的免疫组化染色，既节省时间，又减少了因不同的化学试剂的应用而影响第二种抗原活性的可能性。

RESULTS: Immunoreactivity of proliferating cell nuclear antigenwas present in most ependymal cells in ventricular zone, and PCNA-positive cells were sparsely distributed in the parenchyma in normal and sham-operation groups.

结果：在正常组和假手术组多数脑室室管膜细胞增殖性细胞核抗原免疫反应阳性，脑实质内有零星散在增殖性细胞核抗原阳性细胞。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

LT and ST are the main enterotoxins of enterotoxigenic \$Escherichia coli \$found in clinical isolates, and CS3 (the common antigen in the CFA/Ⅱ family of fimbrial antigens) is one of the most prevalent antigens of colonization factors.

不耐热肠毒素和耐热肠毒素是产肠毒素大肠杆菌的主要致病因素，CS3为该菌的优势定居因子，是定居因子CFA/II菌毛抗原的共有抗原组分。

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

The Sm antigen was found to be quite heterogenous in nature.

Sm抗原有较大的异质性，不同抗Sm血清的相应抗原亦不完全相同。

Objective To estimate the frequencies of HLA-B27, B7, B13 and B40 alleles in Chinese patients with ankylosing spondylitis or related disorders and to analyze heterozygotic frequencies among these loci and the cross-reactivities of their antigens.

目的 检测1194例初诊怀疑患强直性脊柱炎和其它关节性疾病患者的HLA-B27、B7、B13和B40位点抗原的分布，并分析这些位点的相互杂合和其抗原的交叉反应性。

Objective To estimate the frequencies of HLA-B27, B7, B13 and B40 alleles in Chinese patients with ankylosing spondylitis or related disorders and to analyze heterozygotic frequencies among these loci and the cross-reactivities of their antigens.

目的 检测1194例初诊怀疑患强直性脊柱炎和其它关节性减肥疾病治愈患者的HLA-B27、B7、B13和B40位点抗原的分布，并分析这些位点的相互杂合和其抗原的交叉反应性。

This investigation is designed to evaluate the value in comment on the treatment effects of the diagnostic kit made by monoclonal anti idiotypic antibody NP30 of Schistosom japonicum .

为证实用日本血吸虫单克隆抗独特型抗体NP30制备的抗体检测试剂盒考核治疗效果的价值，采用双&抗原&夹心酶联免疫吸附试验，用NP30替代抗原，检测血吸虫病抗体。

Dendritic cell vaccine was impacted and sensitized by tumor associated antigen and antigen polypeptide in vitro, then was retransfused or immunogenically inoculated to tumour carrier host, can evoked specific CTL's antitumour immunoreaction.

应用肿瘤相关抗原或抗原多肽体外冲击致敏DC制成疫苗，回输或免疫接种于载瘤宿主，可诱发特异性CTL的抗肿瘤免疫反应。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。