抗原

Methods the trophozoite antigens were obtained with in vitro culture system of giardia lamblia stile. after further separation, purification and activity verification, the antigens were used to immunize the balb/c mice divided into different groups,then indexes associated with immunity were determined. through challenge infection, the most protective antigenic component of g.intestinalis were selected based on its anti-infection ability.

利用已建立的蓝氏贾第鞭毛虫滋养体体外培养系统，获取滋养体抗原，并进一步分离、纯化、活性鉴定；然后分组免疫小鼠，检测相关免疫指标；再行攻击感染试验，视其抗感染能力，筛选出保护性强的抗原组分。

Experimental mice were exposed to Trypanosome evansi after injection by different doses of antigenic reservoir. The results showed that 20 μg dose produced 83% and 67% protection to 2 sample variations after 4 immunizations. However, 3 immunizing doses could not give good protection. No protective effects were detected when experimental animals immunized by single VSG exposed to isotype from different rabbits.

用不同剂量抗原库免疫小鼠后进行攻虫，结果显示：经四次免疫后，20μg 剂量对随机抽取的两个变异体100 条虫体的保护率分别达到 83%和 67%；改变免疫方法，经三次免疫后的保护效果不理想；用单一 VSG 抗原免疫小鼠，接种来自不同兔体的同型虫体，不能起到相应保护作用。

Objective: To obtain the recombinant truncated porcine zona pellucida protein 3β(tunc- pZP3β) for further research in its function.

目的：制备具有猪卵透明带-3β抗原活性的截短型pZP3β蛋白(tunc-pZP3β)，以便对该蛋白的抗原表位区域作深入研究。

There is no mature scheme on haploidentical non-T-depleted transplantation in China. Prognosis of chemotherapy is poor for refractory and high-risk leukemia. Allogene hematopoietic stem cell transplantation should be applied without delay. If we can go beyond the histocompatibility leukocyte antigen boundary, HLA-haploidentical family members would became the resource of allo-HSCT donors.

目前，国内外尚无单倍型相合未去T细胞移植成熟的方案，对于难治和高危白血病患者，化疗预后是很差的，应不失时机进行异基因造血干细胞移植，如果能够跨越人类主要组织相容性抗原的限制，用人类主要组织相容性抗原半相合的亲属作为造血干细胞移植的供体，就可以彻底解决干细胞来源问题。

ConclusionThe first experiment Immunetolerance in mice sciatic nerve allograft by intrathymic injection of allogene : the specific immune tolerance can be induced in the sciatic nerve allograft by intrathymus injection of MHC allogene.

研究结论：第一部分：小鼠胸腺内注射异基因抗原在同种异体坐骨神经移植免疫耐受中的作用胸腺内注射异基因MHC抗原可诱导异体坐骨神经移植的特异性免疫耐受。

Amido black assay could only marginally distinguish the responsiveness to S-180 membrane and internal antigens.

氨基黑素试验仅稍显著的区分对S-180內部抗原及膜抗原的反应。

Nerve autografting was performed in autograft group, and allogenic antigen was injected into allogenic antigen group following nerve allografting.

异基因抗原注射组在异体神经移植后注入供体异基因抗原。

Methods: Hapten of dimethoate was synthesized, then it was conjugated to bovine sera albumin and ovalbumin to prepare immunogen and coating antigen respectively by carbodiimide method.

采用生产乐果的中间体硫磷醋与l， 4-二氨基丁烷反应合成乐果半抗原，利用碳二亚胺法，将半抗原与牛血清白蛋白及卵清蛋白偶联，分别制备免疫原和包被原。

Three immobilization modes of antigen to the polymers in the pH-sensitive phase separation immunoassay were investigated and compared. The results showed that the immobilization mode in the presence of N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide hydrochloride rendered the most desirable results. The immobilization efficiencies and immunological reaction activities of immobilized antigen of this mode were improved over the other two modes.

本文研究并比较了pH敏感相分离免疫分析中抗原在高分子上的三种固定化模式，结果显示由碳二亚胺偶联的固定模式效果最好，固定化效率、固定抗原的免疫反应活性较之另外两种模式均得到了提高。

Avidin-biotin complex ELISA in detecting the specific antibody in patients infected with Clonorchis sinensis was studied.

生物素一亲和素这一生物反应放大系统引入酶联免疫吸附试验建立的ABC一ELISA方法，经对一些可溶性抗原、抗体检测研究，证明敏感性和呈色反应强度均高于常规ELISA，特别有助于低水平抗原抗体检测〔卜7，。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。