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After immunization, kinetics of antibody response, isotype of IgG antibody, and antibody responses to the individual components of the chimeric antigen were detected by ELISA. Interactions of the antibodies with native antigens located on surface of merozoite of Plasmodium falciparum were analyzed by indirect immunofluorescent assay.

以ELISA检测免疫血清中特异性抗体的动态变化、IgG抗体亚型及对融合抗原各组分的抗体水平,以间接荧光抗体实验分析免疫血清对恶性疟原虫天然抗原的识别情况。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原,评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标,并对检测结果与化学发光免疫分析法(chemiluminescence immunoassay,CLIA)和时间分辨荧光免疫分析法(time-resolved fluorescence immunoassay,TRFIA)进行了相关性分析。

Coil O85 antigen contained 8 varieties of genes, including UDP-Nacetylglucosamine-2-epimerase gene (f1). UDP-galactopyranose mutase gene, glycosyl transferase genes (f3, f5, f6, f8), O-antigen transferase gene and O-antigen polymerase gene, in which two genes, wzx and wzy and 4 pairs of primers were identified to be specific to E.

发现8个开放阅读框架并确定功能,分别为:UDP-N-乙酰葡萄糖-2-异构酶基因,吡喃型UDP-半乳糖变位酶基因,糖基转移酶基因(orf3、orf5、orf6和orf8),O-抗原转运酶基因和O-抗原聚合酶基因。

The results showed that the mycelium supernatant antigen was better than that of exopolysccharides antigen.

实验结果表明,上清液抗原的的免疫效果要好于孢外多糖抗原的免疫效果。

Dramatic enlargement of lymph nodes and other lymphoid tissue such as the adenoids and tonsils is often a normal response to a variety of relatively weak antigenic stimuli such as mild viral and bacterial infections or vaccinations in infants and children, whereas in adults these antigens will not elicit a generalized response.

在婴儿和儿童显著增大的淋巴结或其他的淋巴组织像扁桃体常常是对各种相对弱抗原刺激像轻度病毒和细菌感染或接种疫苗,然而在成人这些抗原将不会引起全是性反应。

By ABC-immunohistochemical technique combined with LBAS- LUZEX-F computer automatic pictural analysis system, we detected the HSV-1 antigens in cornea of HSK rabbit dynamically.

通过对HSK兔三叉神经节病理形态学及定量免疫组织化学研究,首次定量分析了三叉神经节各分支区域神经原内HSV-1抗原分布情况,揭示SCRN抑制病毒抗原,保护神经细胞的作用。

It has been demonstrated that Ag presentation by receptor-mediated internalization of DCs can be enhanced up to 1000-fold, compared with fluid phase Ag pinocytosis.

业已证 实,DC是目前发现的功能最强的抗原提呈细胞,也是唯一能激活初始T细胞的抗原呈递细胞。

DC takes up antigen through phagocytosis, pinocytosis, and endocytosis via different groups of receptor families, such as C-type lectin receptors for antigen capture and presentation, Toll-like receptors for pathogen recognition and DC activation.

DC通过不同的受体吞饮、吞噬和胞吞抗原,例如C型凝集素受体捕获和呈递抗原,通过Ton样受体识别病原体和激活DC。

The results showed that the purified serum can recognize MUC1 protein ..proliferation assays of T-cells from spleen of Balb/C inoculated with the positive phages showed that T-cells were markely proliferated in absence of adjuvant during immunization , which demenstrated that phage might be a proper vector for the immunization.

为证实模拟抗原的免疫原性,进一步用展示MUC1抗原模拟表位的噬菌体阳性克隆免疫小鼠,3次免疫小鼠后,血清(1:1000)中能检测到较强的MUC1抗体阳性;以最大OD450值之纯化血清做为一抗行蛋白印迹分析,结果显示:纯化血清可识别MUC1蛋白;小鼠脾脏淋巴细胞增殖率检测结果表明:噬菌体展示肽表位可在无佐剂存在情况下,大大促进小鼠的脾淋巴细胞的增殖,证明噬菌体是一较好的生物载体。

QS-21, an acylated bidesmosidic triterpenoid saponin isolated byKensil et al in 1992 from the South American tree, Quillaja Saponaria Molina, is a potentimmunological adjuvant It has been shown highly effective in inducing CTL and antigen-specificantibody responses to exogenously added antigens and enhancing both humoral and cell-mediatedimmune responses.

QS-2l是1992年由美国化学家Kensil等人从蔷薇科植物QuillajaSaponria Molina中分离得到的一种带脂肪链的双糖链三萜皂甙,具有极强的免疫辅助活性,能有效诱导外加抗原的胞毒T淋巴细胞的响应及抗原专一性抗体的产生,并能增强细胞的免疫反应。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

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但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。