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So, Z-1 was named IEM106 and regarded as a live oral cholera vaccine candidate eliciting both antibacterial and antitoxic immunity.

以上结果表明,Z-1无毒,免疫家兔可诱导抗菌抗毒免疫,具有很好的保护作用,可以作为霍乱弧菌抗菌抗毒口服活菌苗候选株,命名为IEM106。

Secondly, through the filariasis immune experiment, people improve that the skin is wiped with the levamisole liniment at regular intervals instead of preparing the bacterin for inoculability, levamisole actions of resisting the virus and infection are exerted without the disease when inoculating the pathogeny, and the specificity immune protection is obtained under the pathogeny action, the immune preventive intention is reached just as injecting the bacterin.

二是,经丝虫病免疫实验证实,不用制苗接种,通过左旋咪唑搽剂定期皮肤涂药,在接种病原时,发挥左旋咪唑抗病毒、抗感染作用而不发病,并在病原作用下,获得特异性免疫保护,就像注射疫苗一样达到免疫预防的目的。

The treated group was revaccinated with 5μg YRHBV while the control group with 10μg blood-derived HB vaccine, their anti-HBs response was determined 1,12,24 months after revaccination.

结果显示:用5μg重组酵母乙肝疫苗进行再免疫能取得良好的抗-HBs应答,不论再免疫前抗-HBs水平高低,再免疫后能呈现免疫回忆反应。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物,用低维生素E且添加鱼油日粮饲喂雏鸡,复制脑软化症动物模型,采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标,并对患雏病理组织进行了显微及超微结构观察。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法,采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体,初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法,采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体,初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

The immuneregulating action that briefed on the electropuncture further regulated each other throughthe neural peptide / neurotransmitter, hormone and CK. 6Function laws, which compare point: Dazhui is good at suppressing theinflammation, while Mingmen is strong to the regulating immune function; it is Feixuethat has regulating action too.

比较腧穴的免疫调节作用规律:大椎穴的抗炎治疗作用(如足爪肿胀率、下丘脑 CRH 含量,血清 TNFα含量)较强,命门穴对于免疫调节作用(如脾脏 T、B 淋巴细胞转化率、IL-2 活性、肾上腺 IL-2 含量等)较强,非穴也具有一定的抗炎免疫作用,但弱于大椎穴及命门穴。

Pioneering work by Cohen and coworkers about T cell vaccination in the experimental autoimmune disease, has showed that the establishment of immune response against T cell vaccine, such as anti-idiotype T cells or anti -idiotype antibody, by the way of the upregulation of immune networks, and subsequently functional depression of autoimmune to self-antigen were made by vaccining mice or rate with the attenuated autoreactive T cells .

从研究TCV在防治自身免疫性疾病中的作用机制表明,人为的TCV免疫,建立了拮抗T细胞的免疫应答,使免疫网络产生上调作用,抑制了原有的或即将出现的相关T细胞的功能,由此推测,是否能够把同种反应性T细胞作为TCV,来建立抗同种免疫反应的内环境,诱导抗原特异性的同种免疫耐受或免疫低反应性。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔,按抗原量1.0mg/kg接种于新西兰白兔皮内,间隔10d加强免疫1次,共加强4次后,利用间接ELISA法检测藻酸盐抗体效价:将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上,置入细胞培养箱继续培养3d,分为5组:A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组,分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养,分别于1h、6h、12h和24h不同的时间点,取出96孔培养板,采用MTT法检测5组腮腺腺细胞增殖情况

According to correlative literatures, the diagnosis and treatment of bronchiolitis obliterans syndrome in three patients with hematological malignancies after allo-HSCT were analyzed. The function of haematopoiesis re-established successfully. The acute graft versus host disease did not occur in these patients except one patient occurred grade Ⅱ aGVHD, and the chronic graft versus host disease occurred in the 5th, 6th and 6th months after allo-HSCT respectively, but the cGVHD was recrudescent after treatment. The bronchiolitis obliterans syndrome occurred in the 9th, 7th and 11th months after allo-HSCT respectively, and they received the treatment of immunosuppressive, immune human serum globulin, azithromycin and to breathe in β2 receptor agonist and budesonide.

结合相关文献,对3例恶性血液病患者异基因造血干细胞移植后发生闭塞性细支气管炎综合征的临床特点和诊断治疗进行分析。3例患者移植后造血功能均顺利重建,2例未发生急性移植物抗宿主病,1例发生II度急性移植物抗宿主病;3例患者分别于移植后5,6,6个月发生广泛型慢性移植物抗宿主病,经治疗后病情仍有反复;3例患者分别于移植后9,7,11个月发生闭塞性细支气管炎综合征,给予免疫抑制剂、静脉免疫球蛋白、阿奇霉素及局部使用β2受体激动剂及布地奈德吸入治疗,2例在治疗4周后病情有所改善,另1例病情无改善。

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