抗免疫的

The results show that: This tablet can decrease the rat"s footpad swell induced by Freud"s, inhibit the permeability of the blood capillary in inflammatory joint and decrease the 11-2 level in serum while increasing the rat"s body weight,(2) It can lower the rat"s temperature and the permeability of the blood capillary in inflammatory joint induced by collagen and inhibit the delayed type hypersensitivity,It can lower the stickiness of full blood and the accumulation of erythrocyte obviously inblood stasis model rats,lt can inhibit the formation of hemolysin and decrease the index of carbon granule clearance in mice, It can lower the permeability of celiac blood capillary and inhibit the granulomatous hyperplasia by sc cotton ,It can inhibit the pain induced by acetic acid and raise the pain threshold value of heat stimulation.

结果表明：关络通片能明显降低佐剂性关节炎大鼠左右足趾肿胀度，降低大鼠炎症关节的通透性，抑制其IL-2含量，对大鼠体重增长缓慢有一定的改善作用；能改善Ⅱ型胶原诱导性关节炎大鼠的表征指标及炎症关节的通透性，对其迟发型超敏反应有一定的抑制作用；对血瘀模型大鼠，能显著性降低其全血粘度、血浆粘度、红细胞压积；能抑制小鼠的溶血素抗体生成，抑制小鼠网状内皮系统的吞噬能力，具有一定的抑制机体免疫功能的作用，能抑制小鼠腹腔毛细血管通透性增高，抑制大鼠棉球肉芽组织增生，具有一定的抗炎作用；能抑制小鼠醋酸扭体反应、提高小鼠热板痛阈值，具有一定的镇痛作用。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).

综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

By the affinity chromatography,fusion protein is purificated. The antiserum of chicken and rabbit were prepared with the fusion protein and Ghrelin-KLH.With active immunity and passive immunity,confirmed that Ghrelin of chicken have no obviously impact on ultimum metabolite,plasma Insulin,glucagons, T_3 and T_4.It entrances plasma GH,but inhibit food intake and increase of fat and body weight.These conclusion are obviously different from the mammal.

以纯化的融合蛋白和人工合成的Ghrelin-KLH为抗原，制备了鸡、兔抗血清：通过主动免疫、被动免疫中和实验证实禽类Ghrelin对鸡血浆中代谢产物、胰岛素、胰高血糖素、甲状腺素浓度没有明显的影响，对GH的分泌有促进作用，但与哺乳动物中不同，Ghrelin抑制鸡的脂重、体重的增加，抑制鸡的摄食。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

MethodsThe activity of macrophage cell in abdomen cavity and NK cell in spleen and the SIgA contents in saliva were detected, and the survival rate of mice which had been infected by influenza virus FM 1 were observed after the powder of Le IFN α/supplement material mixture had been insufflated in mice buccal cavity.

一些研究表明，人体的干扰素受体，主要分布在口腔、腭咽及鼻腔黏膜部，只要少量的IFN同上述部位的受体结合，即可发挥IFN的生物活性[1] ，同时也有不少以人的αIFN通过口腔途径给药用于抗病毒和免疫调节的动物实验[2 ] 。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Can reverse JA-induced migration inhibition JA-treated cells also showed significantly higher adhesion to laminin In conclusion these results indicate a novel anti-tumor role of JA via reducing reorganization of cytoskeleton increasing integrin 3 expression and adhesion ability to inhibit tumor cells migration Furthermore immunofluorescence staining of phalloidin/actin indicated that JA-treated cells exhibited cytoskeleton rearrangement and significant decreases in the number and length of filopodia Activity of Rho-family small GTPases was detected by a GST-pull down assay and showed that GTP-loaded Cdc42 and Rac were decreased in JA-treated cells In the future we hope these findings will be helpful to further understand the mechanisms underlying tumor progression in oral cancer and subsequently improve the cancer prevention and treatment

以抗体阻断integrin ？3可以有效使OC2恢复被JA所抑制的迁移能力。细胞贴附实验指出JA能提高OC-2在laminin环境下的贴附能力。当我们进一步以免疫萤光染色观察细胞，发现在JA处理下的OC-2，其外形、细胞骨架和细胞边缘的filopodia的密度和长度明显减少，利用GST-pull down assay侦测到Cdc42-GTP和Rac-GTP的表现量降低。推论爵床素A可能透过刺激口腔癌细胞大量表现integrin ？3，使细胞贴附能力增加或是透过改变细胞骨架来降低细胞迁移能力。希望本研究对於JA所做的抗癌及抗转移效应评估能提供口腔癌治疗在研发上一些帮助。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。