抗免疫的

Experiment in vivo: Establishing the HSE model of Balb/c mouse intracranially to identify effection of glycyrrhizin on mortality of mice with HSE, the replication of HSV-1and expression of iNOS mRNA due to secondary antoimmune-mediated tissue damage.

体内抗病毒实验：经颅内接种HSV-1建立HSE的小鼠模型，观察甘草甜素对HSE小鼠死亡率的影响，在体内对病毒的抑制作用，以及对介导HSE继发性免疫损害的iNOS的mRNA表达的抑制作用。

Here we show that sooty mangabeys have substantially reduced levels of innate immune system activation in vivo during acute and chronic SIV infection and that sooty mangabey plasmacytoid dendritic cells produce markedly less interferon- in response to SIV and other Toll-like receptor 7 and 9 ligands ex vivo.

首先，研究人员发现，与被感染的恒河猴相比，成熟和活化的pDCs（通过形成IFNα和引发适应的免疫响应而扮演了一个关键的抗病毒角色）在被感染的乌白眉猴中含量很低，其中pDCs并没有转移到被感染的乌白眉猴的淋巴结中。

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片，将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片，用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS，并观察该酶在各期虫体内的分布；用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

The journal publishes reviews covers the whole field of the biology and biochemistry of cancer, emphasizing oncogenes and tumor suppressor genes, growth-related cell cycle control signalling, carcinogenesis mechanisms, cell transformation, immunologic control mechanisms, genetics of human cancer, control of cell proliferation, genetic and molecular control of organismic development, rational anti-tumor drug design.

该杂志主要发表有关肿瘤生物学和生物化学的综述。其内容包括：致癌基因和肿瘤抑制基因，与生长有关的细胞周期控制的信号转导，致癌机理，细胞转化，免疫控制机理，人类癌症遗传学，细胞增殖的控制，组织发育的遗传和分子控制，合理的抗肿瘤药物设计等。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Objective: To investigate the survival time of corneal rejection in allogene mice model and the effects of J2 on prevention of allograft rejection; To determine the effect of J2 on the immunal systems of both normal mice and those after allogeneic corneal transplantation.

目的： 研究同种异基因小鼠角膜移植后植片的生存时间及J2的抗角膜排斥反应的作用；研究J2对正常小鼠及同种异基因角膜移植小鼠的免疫系统的影响，从而探讨新型药物J2对于角膜移植排斥反应的作用机制。

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究，比较了雄蚁和雌蚁的异同，并对可能造成这些差异的行为学联系进行了初步的探讨。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

Leitzmann, MD, from the National Institutes of Health in Bethesda, Maryland, and colleagues."Whether dietary zinc intake affects intraprostatic zinc levels is unknown. However, ingestion of 150 mg/day or more of zinc has undesirable metabolic effects, such as immune dysfunction and impaired antioxidant defense that are potentially related to prostate cancer."

Leitzmann博士表示，虽然日常饮食的锌摄取量是否会影响前列腺中的锌含量仍然未知，但是前列腺中拥有较高的锌浓度可能会对於前列腺的健康造成影响，但是，每天服用150mg以上的锌，对於代谢会造成不良影响，如免疫功能障碍并造成与前列腺癌有关的抗氧化剂之缺损。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。