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Methods Animal inflammatory models were established by injection of dimethylbenzene for mice ear swelling, carrageenin for rat footpad, cotton pellet wool for rat granuloma respectively. The content of prostaglandin E2(PGE2) and leukotriene B4(LTB4) from peritoneal macrophages were measured by RIA and RP-HPLC respectively.

采用二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足肿胀、棉球诱导的大鼠肉芽肿模型观察TFC的抗炎作用;分别采用放射免疫法和反相高效液相色谱法检测TFC对大鼠腹腔巨噬细胞产生前列腺素E2(PGE2)和白三烯B4(LTB4)的影响。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中,我们应用免疫组织化学的双标方法,用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞,用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白,分别应用FITC和Cy3标记的荧光二抗来显示,最后用激光扫描共聚焦显微镜进行观察并定量分析。

Objectives To investigate the detection rate of patients with ABO haemolytic disease of the newborn in different groups of patients with haemolytic disease of the newborn .

目的 探讨不同群体新生儿溶血病患者中ABOHDN患者的检出率。方法采用免疫血液学的有关试验,鉴定HDN患者的ABO血型,定性检测HDN患者红细胞上结合的及血液中游离的病理性IgG抗 A。

Therefore, these antibodies have high specificity for CL and could be used in immunological research and application.

因此,所制备的抗盐酸克伦特罗的抗体的特异性高,可用于与其相关的免疫检测研究和应用。

Antigenic protein vaccines contain many CD8~+ T cells epitope and CD4~+T cells epitope, they can be presented by not only MHO I molecule but also MHC-II molecule and active immune response.

蛋白疫苗的特点是含有己知和未知的CD8~+T细胞表位和CD4~+T细胞表位,不被特定的HLA表型所限制,既可通过MHC-Ⅰ类分子途径,又可通过MHC-Ⅱ类分子途径激活抗肿瘤免疫应答,因此采用蛋白疫苗可能具有更大的临床应用意义。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin,OA)免疫豚鼠造成过敏模型,观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响;在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏,观察LMWH对OA引起肥大细胞脱颗粒的影响;以4-氨基吡啶诱发小鼠舔体反应,观察LMWH对舔体反应的影响;以冰醋酸使小鼠毛细血管通透性增加,观察LMWH对通透性的影响。

Mel coule promote the proliferation of PBL and prohibite the apoptosis of PBL from type 2 DM patients, and its effect varied with its concentration; Mel took its greatest influence at the pharmacological concentration, probably due to Mel could regulate the function of immune system and have effect of antioxidation.

Mel有促进2型DM患者外周血淋巴细胞的增殖反应并有效抑制其凋亡的作用,可能与Mel的免疫调节和抗氧化作用有关,为临床应用Mel作为2型DM的辅助用药奠定基础;4。

The Interference of cell cycle and proliferation of BGC-803 and Hela cell by water extraction of Agrimonia is studied by electronic microscopy and flowcytometry respectively, and the IL-12 and TGF-β are also detected by ELISA in order to explore the pharmacological mechanism of Agrimonia.

仙鹤草抗肿瘤研究应用流式细胞技术和电镜技术,在体外分别研究了仙鹤草水提取液对胃癌BGC-803和宫痉癌Hela细胞的生长抑制作用和细胞周期的影响,同时检测对免疫活性因子TGF-β和IL-12表达的影响,阐明其对人癌细胞生长抑制的药理机制。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞,用G〓选择压力筛选,细胞免疫荧光检测表明,pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白,这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

All major neuropil regions are labeled by 5-HT antiserum except the optic lobes, antennal lobes and calyces of mushroom bodies, which are almost devoid of 5-HT immunoreactivity.

除常规的免疫阳性神经元(仅细胞质被标记的神经元和整个胞体都被标记的神经元)外,我们在前脑中部及触角叶内侧还发现了一些只有细胞核对 5-HT 抗血清发生反应的特殊神经元。

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