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METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞,运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化;用gp120处理并用anti-gp120-FITC进行染色,运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况;用抗磷酸化ERK 抗体免疫荧光方法进行染色,运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

blood serums of the crowd are collected, antibody corresponded to the four virus examined by elisa、pa and colloidal gold strip. western blot is the confirmation test of positive for antibody of hiv. result: in the 3852 cases of paid blood donor there are 7 curriers of hiv are detected and they are all paid blood donor in past.

采集有偿献血员的血清,通过酶联免疫吸附实验、明胶颗粒凝集实验和胶体金实验来检测血清中的这四种传染病病原体的相应抗体,抗-hiv阳性确认实验是蛋白印迹实验。

Objectives The study was to investigate the adhesive ability of the intestinal probiotics as living and heat-killed Bifidobacterium to human colon cultured carcinoma cell line HT-29 cells. Methods With microscope the adherence index of the living and heat-killed Bifidobacterium were observed.

双歧杆菌是人体肠道重要的益生菌,对宿主发挥生物屏障、营养、免疫、控制内毒素血症、延缓衰老、抗肿瘤等生理作用,能够防止多种疾病和不良反应的发生,目前已广泛应用于益生菌制剂的研制。

In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune system, thereby triggering cytotoxic effector functions.

另外需说明的是,只有特定的几种鼠源同种型单抗显示出有效结合并激活人类免疫系统,激活细胞毒作用的能力。

Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原,免疫BALB/c小鼠,按常规方法进行融合,间接ELISA方法进行筛选,采用有限稀释法,经过三次克隆,最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5),分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类,三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

The antitumor effect against syngeneic marine colon carcinoma and the immune response in vivo were obviously enhanced by treating them with TCV-4-1BBL.

4-1BBL基因修饰的小鼠结肠癌细胞瘤苗能显著增强小鼠的免疫功能,并能产生显著的抗肿瘤作用。

Biotechnology Research Institute is primarily dedicated to education and research in biotechnology including molecular bio-pharming, subviral RNA pathogens, the metabolism of lipids and lipoproteins, functions and immune suppression, cell division and DNA replication, membrane lipids, antioxidants, molecular-genetic analysis of RNA viruses, blood coagulation.

主要致力于生物技术的教学与研究,研究领域包括分子生物药物化学,亚病毒RNA病原体,脂类和脂蛋白的物质代谢,机能和免疫抑制,细胞分裂和DNA复制,膜脂,抗氧化剂,RNA病毒的分子-遗传分析,血凝固等。

This virus generally seems to cause immune system weakening , and this results in secondary opportunistic infections often arising, for example: bad toothache, ear infections, eye styes, fungal skin infections, urinary-tract infections, etc (all requiring antibiotics or antimicrobials to clear).

这种病毒一般,似乎导致免疫系统削弱,和中学的机会性感染往往产生这样的结果,例如:坏的牙痛,耳部感染,眼睛麦粒肿,皮肤真菌感染,泌尿道感染等(所有需要抗生素或抗菌剂清除)。

Objective: To observe its anti-tumor and immunlolgical regulation activity or Ramuli Cinnamimi and Poriae Decoction and providing its basic theory for clinical application.

目的:研究桂枝茯苓汤体内抗移植性肿瘤的作用及其对机体的免疫调节作用,为桂枝茯苓汤的临床应用提供理论依据。

We chose 113 patients who have complete date of clinic and observe the clinical treatment compared with the medicine of Yi Gan San. The result is that its effective rate is 87.3%, prior to the control group"s 65.5%.(p.01). It"s principle of affect may have something withanti-virus, regulating, immunity, protending liver, improve microcirculation.

并以此为原则创立肝病Ⅰ号,多年来治疗慢性乙型肝炎患者疗效显著,本文选择临床资料齐全的113例患者,并以乙肝散作为对照药物进行了临床治疗的观察,结果发现其有效率为86.9%,明显优于对照组乙肝散的65.5%,(P.01),其作用机理可能与抗病毒、调节免疫、保护肝脏、改善微循环有关。

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然而,要让一个真正的引用,你需要提供详细的个人和财务信息。