抗免疫的

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetle s, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size.

采用树脂石蜡组织包埋连续切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法，首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究，分析了三种甲虫脑组织结构特征，初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

In addition, there is a polyphenol substances "Anthocyanin Trimerization original" experiment confirmed that the strongest anti-viral effect, can effectively inhibit HIV-1 and human immune cell receptor combination, which can prevent the immune system against HIV.

此外，还有一种多酚物质&三聚原花青苷&，实验证实其抗病毒作用最强，能有效抑制 HIV－1 与人体免疫细胞受体的结合，从而可预防 HIV 侵害免疫系统。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

RIG-I overexpression sensitizes hepatocellular carcinoma cells to the cell arrest and apoptosis induction by IFNalphaIn addition to the critical role in the antiviral response, IFNs can also regulate the immune response, induce cell growth inhibition and cell differentiation.

三、RIG-I增强肝癌细胞对干扰素免疫治疗敏感性的研究干扰素(Interferon， IFN)作为一种病毒抑制因子，不仅具有抗病毒作用，而且还具有免疫调节、抑制细胞增殖以及诱导细胞分化等作用。

The affinity constant of the anti-SQ polyclonal antibody 1405~# was 1.22×10~8L/mol. The cross reactivity with other SAs was less than 0.17%. Three hybridoms cell lines secreting monoclonal antibody against SQ were obtained.

同时，以SQ-BSA为免疫原免疫Balb／c小鼠，经融合实验获得了三株分泌抗磺胺喹噁啉单克隆抗体的杂交瘤细胞株SQl_(12)A_(10)，SQ_(26)B_7，SQ_(28)H_7。

Prokaryotic expressed protein products of candidate cDNAs were purified by SDS-PAGE and used as immunogens to prepare murine antisera.

5利用SDS-PAGE分离原核表达产物，并以纯化的表达产物为免疫原免疫小鼠，制备抗血清。

METHODSMice with swelling of the earflaps induced by xylene, proliferation of granulation tissue induced by cotton pellets were treated with ethanol extract of the walnut leaf, and the weight of immune organ was weighed.

方法］采用二甲苯致小鼠耳壳肿胀，植入棉球致小鼠肉芽组织增生及称取免疫器官质量等方法，观察核桃叶乙醇提取物对小鼠抗炎作用及对免疫器官质量的影响。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷