抗免疫的

VCR (0.2 g/mL) and Fe3O4 nano-magnetic ferrofluid have no harmful effects to hematogenic immunologic function. Magnatic ferrofluid can even elevate the function of leucocytes. The results can supply experimental foundation for erythrocytes to capsulate both magnetic ferrofluid and anticancer drug, and also can provide treatment pathway for anticancer drug erythrocyte carrier targeting antitum or immunity.

VCR（0.2 g/mL）和纳米级 Fe3O4 磁流体对血细胞的免疫功能都没有不良影响，甚至磁流体还可以提高血液免疫反应中白细胞的免疫功能，为红细胞包载磁流体和抗癌药物治疗肿瘤提供实验依据，为抗癌药红细胞载体靶向抗肿瘤免疫提供新的治疗途径。

Flow cytometry showed that the proliferation of splenocytes and cytolysis to tumor cells detected in early phase of tumor development were enhanced in the presence of Tim-3, and this anti-tumor effect was further improved by the combined use of Tim-3 with 4-1 BBL.

膜型Tim-3可在免疫启动阶段作为正向免疫调节因子增强抗肿瘤免疫应答，并可与4-1 BBL协同产生更强的抑瘤效应。

Methods: Chitosan/pVAX1-pZP3α nanoparticles was prepared and administered into each experimental mouse on weeks 0,3 and 6 by intranasal and intravaginal respectively, whereas chitosan without DNA was given to control.

制备pVAX1-pZP3α壳聚糖纳米微粒，分别滴鼻和阴道免疫10只BALB/c 小鼠，对照组免疫不含DNA的壳聚糖，第3周和第6周分别加强免疫一次，ELISA法检测小鼠血清、阴道冲洗液中抗pZP3 IgA和IgG抗体

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

For a peptide to act as part of the host defense system or to be of pharmaceutical interest, high antimicrobial activity must be combined by low hemolytic activity. Our results indicated that the antimicrobial activity increased with increasing charge and polar angle, with charge of ＋4 and polar angle of 140° exhibiting the highest antibacterial and membrane permeabilizing activity. Higher hydrophobicity and hydrophobic moment correlated with increased hemolytic activity, thus decreasing selectivity dramatically.

胜肽要扮演宿主免疫机制的一部分或要成为新型态的抗生素不仅要有强大的抗菌活性还需要有高度的选择性，由本实验的结果发现，当抗菌胜肽的正电荷及极性角度增加时，胜肽之抗菌活性会随之增加，当抗菌胜肽的电荷为＋4而极性角度为 140°时，胜肽有最强之抗菌能力及膜渗透活性；而随著胜肽疏水性以及疏水性矩的增加，会增加溶血活性，结果反而降低了抗菌胜肽的选择性。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

vcr (0.2 g/ml) and fe3o4 nano-magnetic ferrofluid have no harmful effects to hematogenic immunologic function.

vcr（0.2 g/ml）和纳米级 fe3o4 磁流体对血细胞的免疫功能都没有不良影响，甚至磁流体还可以提高血液免疫反应中白细胞的免疫功能，为红细胞包载磁流体和抗癌药物治疗肿瘤提供实验依据，为抗癌药红细胞载体靶向抗肿瘤免疫提供新的治疗途径。

In conclusion, the non-specific immunity of Portunus trituberculatus was significantly affected by salinity low 14 and high 36 stress during 12 hours; PSVF2 has more immunity advantages in these non-specific immune factors than that of CPF1 under salinity stress.

总结：低盐和高盐胁迫对三疣梭子蟹的非特异性免疫影响显著且表现出时间效应；在正常和抗盐度胁迫下，筛选蟹F2比普通蟹F1在非特异性免疫因子方面更具免疫优势。

IDO inhibits the proliferation of eukaryotic intracellular pathogens or tumor cells by depriving them of tryptophan. IDO has been targeted for neurological disorders: it causes decrease of serotonin level in the brain resulting depression, and it causes the accumulation of neurotoxic quinolinic acid. IDO plays a key role in the suppression of T cell response and antitumor immune, it induces immune tolerance to embryo and explant.

IDO可通过降低微环境中色氨酸的浓度而达到抑制病原微生物增殖的作用； IDO与神经系统疾病也密切相关，它能降低5-羟色胺的水平而导致抑郁，也可造成脑中喹啉酸等具有神经毒性的代谢产物的累积； IDO在抑制T细胞免疫和抗肿瘤免疫、诱导母胎免疫耐受和移植物免疫耐受中均发挥重要的代谢性免疫调节作用。

objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.

目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-（14c）-葡萄糖&示踪，研究捷安肽素对真菌(1，3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色，研究35天人胚的发育情况。结果在人胚神经管的室管带中，神经元的细胞质和细胞核ngf免疫反应阳性；在中间带，一部分神经元的细胞核ngf免疫反应阳性，另外一部分神经元的细胞核ngf免疫反应阴性，而其突起ngf免疫反应阳性；在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强，神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性，表明ngf可能是诱导神经管分化发育的重要信号分子，提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子；人胚；神经管；发育

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。