抗体

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

To investigate the ability of ADA isozyme expression to discriminatebetweem lymphoid and nonlymphoid cell types in acute leukemia,the gelfiltration with FPLC system was used as micromethod for ADA isozymeanalysis.

为进一步阐明白血病细胞ADA同工酶生化性质，探讨制备ADA同工酶抗体的可能性，我们采用分子筛FPLC和阴离子交换FPLC系统二步法分离纯化健康人血清、单个核细胞和急性粒单核细胞白血病细胞ADA同工酶，获得部分纯化ADA1和ADA2，ADA1纯化71.62倍，活力62.2u/mg pr，ADA2纯化37倍，活力42.8u/mg pr，ADA总回收率44%，2步FPLC层析法酶活力回收率分别为68.4%和81.5%。

These animals,which threshold of auditory nerve compound active potential and cochlear microphonic potential heightening evidently,showed that the amplitude of endolymphatic potential bring down in various degress,which was related to the change of the active of Na+-K+-ATPase and SDH in vascularis stria and endolymphatic sac.

研究证实，同种内耳抗原(isologous inner ear antigen，IIEAg)免疫可造成ASHL动物模型；并行体液与细胞转移免疫均获得成功；针对内耳组织的特异性抗体和淋巴细胞可经体液循环到达内耳局部；内淋巴囊(endolymphatic sac，ES)在其中起着重要作用〔1～4〕。

Methods AD model was established by injecting β amyloid protein into the hippocampus of rat. The rats were killed ten days after injection. The hippocampus sections were made coronally on a freezing microtome.

采用海马注射A淀粉蛋白的方法建立AD模型。10d后对大鼠进行灌注，取脑，冰冻切片，用NGF、BNDF和NT3抗体行免疫组织化学染色。

The results showed that the virus isolation and identification could generate considerably reliable results but the procedure was complicated and costed long time; microtomy fluorescent antibody detection was fast, but the specificity was low and needed to exclude the false positive from the results. Sandwich ELISA detection combined the merits of the former two methods, but it generated the false negative results when the samples were in bad preservation. Compared with the others, RT-PCR was fast, high sensitive and especially useful for detecting the CSFV directly from the samples. The specificity could be raised by using the nested-PCR to detect the CSFV from the simples, especially when the simples were in bad preservation.

结果显示，病毒分离鉴定法准确性较高，诊断比较容易，但存在试验步骤繁琐，所需时间长的缺点；荧光抗体检测法所需时间较短，但需要经验比较丰富人员来判定结果，且存在假阳性结果，特异性比较差；RT-PCR检测法具有快速、敏感等优点，特别是样品保存不理想时更有价值，利用套式PCR可以提高检测的特异性；夹心ELISA检测法具有快速、敏感等优点，但在样品保存不理想时会出现假阴性结果。

MATERIALS AND METHODS: RBC-C3bR, natural killer cell activity and T lymphocyte subsets of 53 patients with gastric cardia carcinoma, 51 gastric cancer and 30 healthy controls were measured by microzyme garland test, MTT-assay and monoclonal anti-human T lymphocyte antibody.

材料与方法：利用酵母菌花环试验、克隆抗体致敏花环法和MTT法对53例贲门癌、51例胃癌患者及30例正常人外周血RBC-3bR阳性率、T细胞CD3、CD4百分率、CD4/CD8比值及自然杀伤细胞活性进行测定。

7Kd. The minium activity of the catalytic antibody was detected to be 1～2μg/ml (10〓M) by the ELISA technique.

纯化后的酶性抗体经ELISA检测，其最低检出活性为1-2μg/ml。

Platelet counts were dropped dramatically in animals that received antibody injection within 4 h, maintained at the mini-mum level for a period of 44 h, started to rebound after 48 h, and reached to the maximum at 144 h (6 days). Final homeostasis reached at approximately 408 h (17 days), following a minor cycle of platelet number fluctuation.

模型小鼠在抗体注射4h血小板数量大幅降低，维持在低水平达44h，48h后开始恢复，至144h（6天）数量达到最高点，其后又经过一个小的降升循环，在408h（14天）后恢复到初始水平。

They then mixed each type of antibody with the 145 peptides formed by chopping up the vaccine protein.

然后，他们每个混合抗体通过切断疫苗蛋白形成的145多肽的类型。

Immobilized on the sensor Au surface by using self-assembled monolayer technology, and then a series of dynamic change

表面抗原和乙肝表面单克隆抗体的结合、解离以及传感器敏感膜的再生等动态变化过程。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。