抗体

The antibody is bound to an inert polymer support ,then exposed to reacts with the antigen at a different epitope is added. The second antibody used is one that has an enzyme attached to it that coverts a colorless or nonfluorescent substrate into a colored or fluorescent product. The amount of second antibody bound, and hence the amount of protein antigen present in the original sample, is determined by quantification of the intensity of color or fluorescence produced.

将抗体结合在一惰性聚合物载体上，然后与样品接触，洗脱未经结合的蛋白质加入与抗原其他表位也可以结合的另一种抗体，在所用的第二抗体上结合有酶，此酶可把无色的或无荧光的底物转化有色或显示荧光的产物。

In conclusion, 12 avian specific monoclonal antibodies against Pasteurella multocida were obtained in this study. Certain of them provided good passive protection in mice model.

总结实验，以ATCC 12945免疫小鼠后所生产之单株抗体，在抗体特性分析试验中，发现单株抗体能专一的辨认部分巴斯德杆菌之禽分离株。

LL2 is a murine IgG2a antibody to the CD_(22) antigen,We observed its efficacy and toxicity of ~(131)I-labelled-LL2 in the treatment of patients with recrudescent non-Hodgkin lymphoma.

LL2是鼠源性抗CD22抗体，通过131I标记LL2用于复发的非霍奇金淋巴瘤的治疗，观察其疗效及毒副作用。Epratuzumab是人源化的LL2抗体，通过对套细胞淋巴瘤的Ⅰ/Ⅱ期临床试验研究，其有效率为18%，不产生人抗鼠抗体。

THE CORRELATION OF ANTITROPHOBLAST ANTIBODY, EMAB AND HCG-AB WITH IVF-ET PREGNANCY OUTCOMEYANG ZONG-ZHI, SONG DONG-PO, ZOU SHU-HUA, et al（Medical and Health Care Center For Women and Childen of Qingdao, Qingdao 266011, China）［ABSTRACT］ObjectiveTo assess the effect of antitrophoblast antibody, EmAb and HCG-Ab on in vitro fertilization and embryo transfer outcome.MethodsThis study included 140 infertility women of unexplained reasons, salpingemphraxis and/or male factor undergoing IVF-ET, of whom, 31 with repeated failure of implantation; 26 with biochemical loss of pregnancy; 18 with clinical pregnancy loss. Sixty-five with clinical pregnancy served as control group.

目的 探讨抗滋养细胞膜抗体、抗子宫内膜抗体、抗HCG抗体对体外受精-胚胎移植妊娠结局的影响方法选择2002年1月～2007年7月在本生殖医学中心由于不明原因性不育、女方输卵管因素和男方轻度少弱精子症因素不育而接受IVF-ET治疗的妇女140例，其中次植失败31例，生化妊娠丢失26例及临床妊娠丢失18例，持续妊娠65例。

Because ouabain polyclonal antibody made blood pressure reduced for shorter time and possessed slightly allergy, we aimed to prepare a new depressor, ScFv, with high specificness by advanced phage display technique. The heavy-chain and kappa light-chain variable region gene repertoir of immunoglobin were amplified individually with heavy chain and light chain primers of Pharmacia Corporation and assembled into ScFv by a linker with overlap extension PCR.

针对哇巴因多克隆抗体存在的降压时间持续较短及存在轻微的过敏反应等缺点，本研究利用先进的噬菌体抗体库技术，拟研制开发一种具有高度选择性的降压新药--哇巴因单链抗体。

To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, le...

利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库，从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等，尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面，具有广阔的应用前景。

MethodsACCP antibody and RF were detected in serum samples of 90 RA patients, 30 other diseases patients and 35 healthy blood donors.The sensitivity,specificity,positive predictive value, negative predictive value, agreement rate and Youden's index of these parsmeters for the diangnosis of RA were analyzed.

方法对90例RA患者、30例其他疾病患者和35例健康对照组血清用ELISA法检测抗CCP抗体、免疫散射比浊法检测RF，比较2种抗体单独或联合检测对诊断RA的敏感性、特异性、阳性预测值、阴性预测值、总符合率及Youden指数；探讨抗CCP抗体和RF的相关性，通过绘制ROC曲线，计算ROC曲线下面积。

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently.

利用过氧化氢的氧化作用参与抗原抗体反应，使细胞的膜通透性增强，利于荧光标记抗体染液渗入，从而更快速、广泛地接触抗原，更有效的抗原抗体的嵌合交联。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

Morphologic findings of thymuses from 32 anti-acetylcholine receptor-negatie myasthenia grais patients, 12 with and 20 without antibodies against the muscle-specific kinase, were compared with those from 30 AChR-positie subjects.

Lauriola等研究了32名乙酰胆碱受体抗体阴性的胸腺形态学变化，其中有12例肌肉特异性激酶抗体阳性，20例阴性；对照组为30例乙酰胆碱受体抗体阳性的病人。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。